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    Crystallization of recombinant Leishmania major pteridine reductase 1 (PTR1)

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    Authors
    Gourley, David G.
    Luba, James
    Hardy, Larry W.
    Beverley, Stephen M.
    Hunter, William N.
    UMass Chan Affiliations
    Department of Pharmacology and Molecular Toxicology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    1999-09-18
    Keywords
    Animals; Bacterial Proteins; Crystallization; Leishmania major; Oxidoreductases; Recombinant Proteins; X-Ray Diffraction
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://doi.org/10.1107/S0907444999008999
    Abstract
    The enzyme pteridine reductase (PTR1) has recently been discovered in the protozoan parasite Leishmania and validated as a target for therapeutic intervention. PTR1 is responsible for the salvage of pteridines and also contributes to antifolate drug resistance. Structural analysis, in combination with ongoing biochemical characterization will assist the elucidation of the structure-activity relationships of this important enzyme and support a structure-based approach to discover novel inhibitors. Recombinant L. major PTR1 has been purified from an Escherichia coli expression system and used in crystallization experiments. Orthorhombic crystals have been obtained and data to 2.8 A has been measured. The space group is P2(1)2(1)2 or P2(1)2(1)2(1) with unit-cell dimensions of a = 103.9, b = 134.7, c = 96.2 A. One homotetramer, of molecular mass approximately 120 kDa, probably constitutes the asymmetric unit and gives a Matthews coefficient, V(m), of 2.8 A(3) Da(-1) and 56% solvent volume. Self-rotation function calculations show a single well defined non-crystallographic twofold axis with features that might represent additional elements of non-crystallographic symmetry. The detail of exactly what constitutes the asymmetric unit will be resolved by structure determination.
    Source

    Acta Crystallogr D Biol Crystallogr. 1999 Sep;55(Pt 9):1608-10.

    DOI
    10.1107/S0907444999008999
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/33743
    PubMed ID
    10489462
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    Link to article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1107/S0907444999008999
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