Pteridine reductase mechanism correlates pterin metabolism with drug resistance in trypanosomatid parasites
Authors
Gourley, David G.Schuttelkopf, Alexander W.
Leonard, Gordon A.
Luba, James
Hardy, Larry W.
Beverley, Stephen M.
Hunter, William N.
UMass Chan Affiliations
Graduate School of Biomedical SciencesDocument Type
Journal ArticlePublication Date
2001-05-25Keywords
Amino Acid Sequence; Animals; Binding Sites; Crystallography, X-Ray; Dihydropteridine Reductase; *Drug Resistance; Folic Acid Antagonists; Hydrogen Bonding; Leishmania major; Methotrexate; Models, Molecular; Molecular Sequence Data; NADP; Oxidation-Reduction; Protein Structure, Secondary; Pterins; Selenomethionine; Substrate Specificity; Tetrahydrofolate DehydrogenaseLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Pteridine reductase (PTR1) is a short-chain reductase (SDR) responsible for the salvage of pterins in parasitic trypanosomatids. PTR1 catalyzes the NADPH-dependent two-step reduction of oxidized pterins to the active tetrahydro-forms and reduces susceptibility to antifolates by alleviating dihydrofolate reductase (DHFR) inhibition. Crystal structures of PTR1 complexed with cofactor and 7,8-dihydrobiopterin (DHB) or methotrexate (MTX) delineate the enzyme mechanism, broad spectrum of activity and inhibition by substrate or an antifolate. PTR1 applies two distinct reductive mechanisms to substrates bound in one orientation. The first reduction uses the generic SDR mechanism, whereas the second shares similarities with the mechanism proposed for DHFR. Both DHB and MTX form extensive hydrogen bonding networks with NADP(H) but differ in the orientation of the pteridine.Source
Nat Struct Biol. 2001 Jun;8(6):521-5. Link to article on publisher's siteDOI
10.1038/88584Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33744PubMed ID
11373620Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/88584