Genetic requirements for inheritance of RNAi in C. elegans
| dc.contributor.author | Grishok, Alla | |
| dc.contributor.author | Tabara, Hiroaki | |
| dc.contributor.author | Mello, Craig C. | |
| dc.date | 2022-08-11T08:08:58.000 | |
| dc.date.accessioned | 2022-08-23T16:14:00Z | |
| dc.date.available | 2022-08-23T16:14:00Z | |
| dc.date.issued | 2000-03-31 | |
| dc.date.submitted | 2008-09-17 | |
| dc.identifier.citation | <p>Science. 2000 Mar 31;287(5462):2494-7.</p> | |
| dc.identifier.issn | 0036-8075 (Print) | |
| dc.identifier.doi | 10.1126/science.287.5462.2494 | |
| dc.identifier.pmid | 10741970 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/33756 | |
| dc.description.abstract | In Caenorhabditis elegans, the introduction of double-stranded RNA triggers sequence-specific genetic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent. | |
| dc.language.iso | en_US | |
| dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10741970&dopt=Abstract">Link to article in PubMed</a></p> | |
| dc.relation.url | https://doi.org/10.1126/science.287.5462.2494 | |
| dc.subject | Animals; Caenorhabditis elegans; *Caenorhabditis elegans Proteins; Crosses, Genetic; DNA Transposable Elements; Female; *Gene Silencing; *Genes, Helminth; Helminth Proteins; Hermaphroditism; Male; Mutation; Phenotype; RNA, Double-Stranded; RNA, Helminth | |
| dc.subject | Life Sciences | |
| dc.subject | Medicine and Health Sciences | |
| dc.title | Genetic requirements for inheritance of RNAi in C. elegans | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Science (New York, N.Y.) | |
| dc.source.volume | 287 | |
| dc.source.issue | 5462 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/417 | |
| dc.identifier.contextkey | 632027 | |
| html.description.abstract | <p>In Caenorhabditis elegans, the introduction of double-stranded RNA triggers sequence-specific genetic interference (RNAi) that is transmitted to offspring. The inheritance properties associated with this phenomenon were examined. Transmission of the interference effect occurred through a dominant extragenic agent. The wild-type activities of the RNAi pathway genes rde-1 and rde-4 were required for the formation of this interfering agent but were not needed for interference thereafter. In contrast, the rde-2 and mut-7 genes were required downstream for interference. These findings provide evidence for germ line transmission of an extragenic sequence-specific silencing factor and implicate rde-1 and rde-4 in the formation of the inherited agent.</p> | |
| dc.identifier.submissionpath | gsbs_sp/417 | |
| dc.contributor.department | Department of Cell Biology | |
| dc.contributor.department | Program in Molecular Medicine | |
| dc.contributor.department | Graduate School of Biomedical Sciences | |
| dc.source.pages | 2494-7 |