EspFU, a type III-translocated effector of actin assembly, fosters epithelial association and late-stage intestinal colonization by E. coli O157:H7
AuthorsRitchie, Jennifer M.
Brady, Michael John
Riley, Kathleen N.
Ho, Theresa Deland
Campellone, Kenneth Geno
Herman, Ira M.
Waldor, Matthew K.
Leong, John M.
Student AuthorsMichael John Brady
UMass Chan AffiliationsDepartment of Molecular Genetics and Microbiology
Document TypeJournal Article
KeywordsActins; Animals; Animals, Newborn; Bacterial Adhesion; Carrier Proteins; Escherichia coli O157; Escherichia coli Proteins; Hela Cells; Humans; Intestinal Mucosa; Rabbits; Swine; Time Factors
Medicine and Health Sciences
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AbstractEnterohaemorrhagic Escherichia coli (EHEC) O157:H7 induces filamentous actin-rich 'pedestals' on intestinal epithelial cells. Pedestal formation in vitro requires translocation of bacterial effectors into the host cell, including Tir, an EHEC receptor, and EspF(U), which increases the efficiency of actin assembly initiated by Tir. While inactivation of espF(U) does not alter colonization in two reservoir hosts, we utilized two disease models to explore the significance of EspF(U)-promoted actin pedestal formation. EHECDeltaespF(U) efficiently colonized the rabbit intestine during co-infection with wild-type EHEC, but co-infection studies on cultured cells suggested that EspF(U) produced by wild-type bacteria might have rescued the mutant. Significantly, EHECDeltaespF(U) by itself was fully capable of establishing colonization at 2 days post inoculation but unlike wild type, failed to expand in numbers in the caecum and colon by 7 days. In the gnotobiotic piglet model, an espF(U) deletion mutant appeared to generate actin pedestals with lower efficiency than wild type. Furthermore, aggregates of the mutant occupied a significantly smaller area of the intestinal epithelial surface than those of the wild type. Together, these findings suggest that, after initial EHEC colonization of the intestinal surface, EspF(U) may stabilize bacterial association with the epithelial cytoskeleton and promote expansion beyond initial sites of infection.
SourceCell Microbiol. 2008 Apr;10(4):836-47. Epub 2007 Dec 6. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/33765
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