The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing
dc.contributor.author | Miele, Angela | |
dc.contributor.author | Medina, Ricardo F. | |
dc.contributor.author | Van Wijnen, Andre J. | |
dc.contributor.author | Stein, Gary S. | |
dc.contributor.author | Stein, Janet L. | |
dc.date | 2022-08-11T08:08:58.000 | |
dc.date.accessioned | 2022-08-23T16:14:04Z | |
dc.date.available | 2022-08-23T16:14:04Z | |
dc.date.issued | 2007-06-20 | |
dc.date.submitted | 2008-09-22 | |
dc.identifier.citation | J Cell Biochem. 2007 Sep 1;102(1):136-48. <a href="http://dx.doi.org/10.1002/jcb.21284">Link to article on publisher's site</a> | |
dc.identifier.issn | 0730-2312 (Print) | |
dc.identifier.doi | 10.1002/jcb.21284 | |
dc.identifier.pmid | 17577209 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/33775 | |
dc.description.abstract | HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4 genes. HiNF-P interacts with the global histone gene regulator and direct cyclin E/CDK2 substrate p220(NPAT) to potently upregulate histone H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. To gain insight into the function of HiNF-P in a broader cellular context, we performed a yeast two-hybrid screen to identify its novel interacting proteins. In this study, we detected 67 candidate HiNF-P interacting proteins of varying cellular functions. We have identified multiple RNA associated proteins, including the splicing co-factor SRm300. HiNF-P and SRm300 interact in yeast two-hybrid, co-immunoprecipitation, and co-immunofluorescence assays. Our screen also identified several gene regulators that associate with HiNF-P including THAP7. HiNF-P and THAP7 interact in mammalian cells and THAP7 abrogates HiNF-P/p220 mediated activation of histone H4 gene transcription, consistent with its known role as a transcriptional repressor. Finally, we identified several proliferation related proteins including Ki-67 and X transactivated protein 2 (XTP2) which may be functioning with HiNF-P in cell cycle regulation. The HiNF-P interactome indicates that HiNF-P is a multifunctional gene regulator with a large functional network and roles beyond cell cycle-dependent histone gene regulation. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17577209&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1002/jcb.21284 | |
dc.subject | Cell Cycle; Cell Line, Tumor; Cell Proliferation; Chromosomal Proteins, Non-Histone; *Gene Expression Regulation; Humans; Ki-67 Antigen; *RNA Processing, Post-Transcriptional; RNA-Binding Proteins; Repressor Proteins; Transcription, Genetic; Two-Hybrid System Techniques | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | The interactome of the histone gene regulatory factor HiNF-P suggests novel cell cycle related roles in transcriptional control and RNA processing | |
dc.type | Journal Article | |
dc.source.journaltitle | Journal of cellular biochemistry | |
dc.source.volume | 102 | |
dc.source.issue | 1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/436 | |
dc.identifier.contextkey | 635311 | |
html.description.abstract | <p>HiNF-P is a recently identified histone H4 subtype specific transcriptional regulator that associates with the conserved cell cycle control element in the proximal promoter regions of histone H4 genes. HiNF-P interacts with the global histone gene regulator and direct cyclin E/CDK2 substrate p220(NPAT) to potently upregulate histone H4 gene transcription at the G1/S phase transition in response to cyclin E/CDK2 signaling. To gain insight into the function of HiNF-P in a broader cellular context, we performed a yeast two-hybrid screen to identify its novel interacting proteins. In this study, we detected 67 candidate HiNF-P interacting proteins of varying cellular functions. We have identified multiple RNA associated proteins, including the splicing co-factor SRm300. HiNF-P and SRm300 interact in yeast two-hybrid, co-immunoprecipitation, and co-immunofluorescence assays. Our screen also identified several gene regulators that associate with HiNF-P including THAP7. HiNF-P and THAP7 interact in mammalian cells and THAP7 abrogates HiNF-P/p220 mediated activation of histone H4 gene transcription, consistent with its known role as a transcriptional repressor. Finally, we identified several proliferation related proteins including Ki-67 and X transactivated protein 2 (XTP2) which may be functioning with HiNF-P in cell cycle regulation. The HiNF-P interactome indicates that HiNF-P is a multifunctional gene regulator with a large functional network and roles beyond cell cycle-dependent histone gene regulation.</p> | |
dc.identifier.submissionpath | gsbs_sp/436 | |
dc.contributor.department | Department of Cell Biology | |
dc.contributor.department | Graduate School of Biomedical Sciences | |
dc.source.pages | 136-48 |