Nitrite does not provide additional protection to thrombolysis in a rat model of stroke with delayed reperfusion
Henning, Erica C.
Pluta, Ryszard M.
Latour, Lawrence L.
Merrill, Marsha J.
Oldfield, Edward H.
UMass Chan AffiliationsGraduate School of Biomedical Sciences
Document TypeJournal Article
KeywordsAnimals; Disease Models, Animal; Magnetic Resonance Imaging; Male; Nitrites; Rats; Rats, Sprague-Dawley; *Reperfusion Injury; Stroke; Survival Rate; Thrombolytic Therapy; Treatment Outcome
Medicine and Health Sciences
MetadataShow full item record
AbstractAn adjuvant therapy to prolong the therapeutic window for stroke patients is urgently needed. This randomized, blinded, placebo-controlled study investigated adjuvant intravenous sodium nitrite with recombinant tissue plasminogen activator (rtPA) in middle cerebral artery occlusion (MCAO) with 6 and 2 h of ischemia followed by reperfusion in Sprague-Dawley rats (n=59). Quantitative diffusion, T(1)-, T(2)-weighted, and semiquantitative perfusion imaging were performed before and after reperfusion and at 48 h after ischemia to determine the spatiotemporal evolution of stroke. After 48 h animals were killed and examined to evaluate infarct size and evidence of hemorrhagic transformation. Factor VIII immunostaining was performed to assess vessel morphology. Nitrite treatment (6 h group: 37.5 micromol for more than 90 mins; 2 h group: 26.25 and 1.75 micromol for more than 60 mins) did not reduce infarct volume 48 h after MCAO compared with saline-treated placebo groups after 6 or 2 h of MCAO. Stroke progression from baseline to 48 h, based on the apparent diffusion coefficient and relative cerebral blood flow deficits before and after reperfusion and T(2)-weighted hyperintensity at 48 h, did not differ between treated and control animals. These results suggest that nitrite is not a protective adjuvant therapy to delayed rtPA administration after ischemic stroke in rats.
SourceJ Cereb Blood Flow Metab. 2008 Mar;28(3):482-9. Epub 2007 Aug 8. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/33783
Related ResourcesLink to Article in PubMed