Authors
Amrani, NadiaDong, Shuyun
He, Feng
Ganesan, Robin
Ghosh, Shubhendu
Kervestin, Stephanie
Li, Chunfang
Mangus, David A.
Spatrick, Phyllis
Jacobson, Allan
UMass Chan Affiliations
Department of Molecular Genetics and MicrobiologyGraduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2005-10-26Keywords
Life Sciences
Metadata
Show full item recordAbstract
NMD (nonsense-mediated mRNA decay) is a cellular quality-control mechanism in which an otherwise stable mRNA is destabilized by the presence of a premature termination codon. We have defined the set of endogenous NMD substrates, demonstrated that they are available for NMD at every round of translation, and showed that premature termination and normal termination are not equivalent biochemical events. Premature termination is aberrant, and its NMD-stimulating defects can be reversed by the presence of tethered poly(A)-binding protein (Pab1p) or tethered eRF3 (eukaryotic release factor 3) (Sup35p). Thus NMD appears to be triggered by a ribosome's failure to terminate adjacent to a properly configured 3'-UTR (untranslated region), an event that may promote binding of the UPF/NMD factors to stimulate mRNA decapping.Source
Biochem Soc Trans. 2006 Feb;34(Pt 1):39-42. Link to article on publisher's siteDOI
10.1042/BST20060039Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33798PubMed ID
16246174Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1042/BST20060039