Name:
Publisher version
View Source
Access full-text PDFOpen Access
View Source
Check access options
Check access options
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyDocument Type
Journal ArticlePublication Date
2003-06-28Keywords
Animals; Drosophila; Embryo, Nonmammalian; *Genetic Techniques; *RNA InterferenceLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Double-stranded RNA (dsRNA) triggers the destruction of mRNA sharing sequence with the dsRNA, a phenomenon termed RNA interference (RNAi). The dsRNA is converted by endonucleolytic cleavage into 21- to 23-nt small interfering RNAs (siRNAs), which direct a multiprotein complex, the RNA-induced silencing complex to cleave RNA complementary to the siRNA. RNAi can be recapitulated in vitro in lysates of syncytial blastoderm Drosophila embryos. These lysates reproduce all of the known steps in the RNAi pathway in flies and mammals. Here we explain how to prepare and use Drosophila embryo lysates to dissect the mechanism of RNAi.Source
Methods. 2003 Aug;30(4):330-6.
DOI
10.1016/S1046-2023(03)00052-5Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33803PubMed ID
12828947Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/S1046-2023(03)00052-5