Human peripheral blood xenografts in the SCID mouse: characterization of immunologic reconstitution
Authors
Hesselton, RuthAnn M.Koup, Richard A.
Cromwell, Mary A.
Graham, Barney S.
Johns, Michael
Sullivan, John L.
UMass Chan Affiliations
Department of PediatricsProgram in Immunology and Virology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1993-09-01Keywords
Age Factors; Animals; Antigens, CD3; Antigens, CD4; Antigens, CD8; Blood Cells; Humans; Immune System; Immunoglobulins; Leukocytes, Mononuclear; Mice; Mice, SCID; Models, Biological; Peritoneal Cavity; Phenotype; Receptors, Interleukin-2; Species Specificity; Specific Pathogen-Free Organisms; Spleen; Time Factors; Transplantation, HeterologousLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Immune reconstitutions (hu-PBL-SCID mice) resulting from adoptive transfer of human peripheral blood mononuclear cells into 1800 C.B-17 scid-/scid-mice were characterized. Over 90% of reconstitutions were successful as evidenced by human immunoglobulin production. Variability was noted with donor, cell number, and cell type. Human cells (T lymphocytes, few B cells) could be recovered by 5 days after engraftment. High levels of soluble CD8 and interleukin-2 receptors were detected in sera of hu-PBL-SCID mice. Cells recovered from 17 mice proliferated in response to antigens to which the donor had been primed; responses to nonboosted antigen also increased in some animals. After reconstitution, lymphocytes were found in the spleen and lymph nodes without full restoration of normal architecture. The hu-PBL-SCID mouse shows promise as a model system for a variety of immunologic studies. The inherent variation in the system must be minimized for appropriate use of the model.Source
J Infect Dis. 1993 Sep;168(3):630-40.
DOI
10.1093/infdis/168.3.630Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33835PubMed ID
8354904Related Resources
ae974a485f413a2113503eed53cd6c53
10.1093/infdis/168.3.630