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dc.contributor.authorJackson, Rebecca A.
dc.contributor.authorMurali, Sadasivam
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorStein, Gary S.
dc.contributor.authorNurcombe, Victor
dc.contributor.authorCool, Simon M.
dc.date2022-08-11T08:08:59.000
dc.date.accessioned2022-08-23T16:14:39Z
dc.date.available2022-08-23T16:14:39Z
dc.date.issued2006-10-20
dc.date.submitted2008-10-09
dc.identifier.citationJ Cell Physiol. 2007 Jan;210(1):38-50. <a href="http://dx.doi.org/10.1002/jcp.20813">Link to article on publisher's site</a>
dc.identifier.issn0021-9541 (Print)
dc.identifier.doi10.1002/jcp.20813
dc.identifier.pmid17051597
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33913
dc.description.abstractThe transcription factor Runx2 can be controlled by a number of upstream regulators involved in intracellular signalling, including the activation ERK1/2 signaling by fibroblast growth factor-2 (FGF-2). FGFs interact with their cell surface receptors (FGFRs) through an obligate cross-binding interaction with heparan sulfate proteoglycan (HSPG) co-receptors; exogenous HS sugar chains have been shown to potently modulate changes in cell phenotype depending on the stage of tissue differentiation when the HS is harvested, suggesting that HS chain structure and function varies depending on the stage of cell maturity. This study examined the potential of bone-derived heparan sulfate (HS), harvested from differentiating osteoblasts, for the enhancement of preosteoblast growth and differentiation. HS was harvested from conditioned media, cell surface and matrix compartments of postconfluent (differentiating) MC3T3-E1 osteoblasts and dosed back onto preconfluent MC3T3-E1 cells. We show that HS can increase the expression Runx2, ALP, and OPN in preosteoblast cells, suggesting the potential for exogenous HS to shift cells from proliferative to differentiative phenotypes. In line with their structural differences, only HS released into the media was found to co-stimulate the mitogenic effect of FGF-2, whilst exogenous application of all the HSs together with FGF-2 served to increase the expression of OPN. Only the application of cell surface-derived HS triggered a synergistic increase in FGFR1 expression together with FGF-2, although all three HS preparations could trigger transient increases in PI3K, ERK1/2, and stat3 phosphorylation levels. These findings demonstrate that the compartmentally distinct HS species expressed by differentiating MC3T3-E1 cells act in complex ways to coordinate the extracellular conditions that lead to osteoblast differentiation, with the cell surface species coordinating the FGF response.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=17051597&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/jcp.20813
dc.subject1-Phosphatidylinositol 3-Kinase; Alkaline Phosphatase; Animals; Butadienes; Cell Differentiation; Cell Line; Cell Proliferation; Core Binding Factor Alpha 1 Subunit; Culture Media, Conditioned; Dose-Response Relationship, Drug; Extracellular Signal-Regulated MAP Kinases; Fibroblast Growth Factor 2; Heparitin Sulfate; MAP Kinase Signaling System; Mice; Nitriles; Osteoblasts; Osteopontin; Phenotype; Phosphorylation; Protein Kinase Inhibitors; Receptor, Fibroblast Growth Factor, Type 1; STAT3 Transcription Factor; Signal Transduction; Time Factors; Up-Regulation
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleHeparan sulfate regulates the anabolic activity of MC3T3-E1 preosteoblast cells by induction of Runx2
dc.typeJournal Article
dc.source.journaltitleJournal of cellular physiology
dc.source.volume210
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/568
dc.identifier.contextkey646753
html.description.abstract<p>The transcription factor Runx2 can be controlled by a number of upstream regulators involved in intracellular signalling, including the activation ERK1/2 signaling by fibroblast growth factor-2 (FGF-2). FGFs interact with their cell surface receptors (FGFRs) through an obligate cross-binding interaction with heparan sulfate proteoglycan (HSPG) co-receptors; exogenous HS sugar chains have been shown to potently modulate changes in cell phenotype depending on the stage of tissue differentiation when the HS is harvested, suggesting that HS chain structure and function varies depending on the stage of cell maturity. This study examined the potential of bone-derived heparan sulfate (HS), harvested from differentiating osteoblasts, for the enhancement of preosteoblast growth and differentiation. HS was harvested from conditioned media, cell surface and matrix compartments of postconfluent (differentiating) MC3T3-E1 osteoblasts and dosed back onto preconfluent MC3T3-E1 cells. We show that HS can increase the expression Runx2, ALP, and OPN in preosteoblast cells, suggesting the potential for exogenous HS to shift cells from proliferative to differentiative phenotypes. In line with their structural differences, only HS released into the media was found to co-stimulate the mitogenic effect of FGF-2, whilst exogenous application of all the HSs together with FGF-2 served to increase the expression of OPN. Only the application of cell surface-derived HS triggered a synergistic increase in FGFR1 expression together with FGF-2, although all three HS preparations could trigger transient increases in PI3K, ERK1/2, and stat3 phosphorylation levels. These findings demonstrate that the compartmentally distinct HS species expressed by differentiating MC3T3-E1 cells act in complex ways to coordinate the extracellular conditions that lead to osteoblast differentiation, with the cell surface species coordinating the FGF response.</p>
dc.identifier.submissionpathgsbs_sp/568
dc.contributor.departmentDepartment of Cell Biology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages38-50


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