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dc.contributor.authorAshraf, Shovon Imtiaz
dc.contributor.authorIp, Y. Tony
dc.date2022-08-11T08:08:59.000
dc.date.accessioned2022-08-23T16:14:45Z
dc.date.available2022-08-23T16:14:45Z
dc.date.issued2001-12-04
dc.date.submitted2008-07-02
dc.identifier.citationDevelopment. 2001 Dec;128(23):4757-67.
dc.identifier.issn0950-1991 (Print)
dc.identifier.pmid11731456
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33937
dc.description.abstractDelaminated neuroblasts in Drosophila function as stem cells during embryonic central nervous system development. They go through repeated asymmetric divisions to generate multiple ganglion mother cells, which divide only once more to produce postmitotic neurons. Snail, a zinc-finger transcriptional repressor, is a pan-neural protein, based on its extensive expression in neuroblasts. Previous results have demonstrated that Snail and related proteins, Worniu and Escargot, have redundant and essential functions in the nervous system. We show that the Snail family of proteins control central nervous system development by regulating genes involved in asymmetry and cell division of neuroblasts. In mutant embryos that have the three genes deleted, the expression of inscuteable is significantly lowered, while the expression of other genes that participate in asymmetric division, including miranda, staufen and prospero, appears normal. The deletion mutants also have much reduced expression of string, suggesting that a key component that drives neuroblast cell division is abnormal. Consistent with the gene expression defects, the mutant embryos lose the asymmetric localization of prospero RNA in neuroblasts and lose the staining of Prospero protein that is normally present in ganglion mother cells. Simultaneous expression of inscuteable and string in the snail family deletion mutant efficiently restores Prospero expression in ganglion mother cells, demonstrating that the two genes are key targets of Snail in neuroblasts. Mutation of the dCtBP co-repressor interaction motifs in the Snail protein leads to reduction of the Snail function in central nervous system. These results suggest that the Snail family of proteins control both asymmetry and cell division of neuroblasts by activating, probably indirectly, the expression of inscuteable and string.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11731456&dopt=Abstract ">Link to article in PubMed</a>
dc.relation.urlhttp://dev.biologists.org/content/128/23/4757.long
dc.subjectAnimals; Body Patterning; Cell Cycle; Cell Division; Central Nervous System; Cytoskeletal Proteins; DNA-Binding Proteins; Drosophila; *Drosophila Proteins; Gene Deletion; Gene Expression Regulation, Developmental; *Genes, Insect; In Situ Hybridization; Nerve Tissue Proteins; Neurons; Neuropeptides; Nuclear Proteins; Phosphoprotein Phosphatases; *Protein Tyrosine Phosphatases; RNA, Messenger; Stem Cells; Transcription Factors
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleThe Snail protein family regulates neuroblast expression of inscuteable and string, genes involved in asymmetry and cell division in Drosophila
dc.typeJournal Article
dc.source.journaltitleDevelopment (Cambridge, England)
dc.source.volume128
dc.source.issue23
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/59
dc.identifier.contextkey542471
html.description.abstract<p>Delaminated neuroblasts in Drosophila function as stem cells during embryonic central nervous system development. They go through repeated asymmetric divisions to generate multiple ganglion mother cells, which divide only once more to produce postmitotic neurons. Snail, a zinc-finger transcriptional repressor, is a pan-neural protein, based on its extensive expression in neuroblasts. Previous results have demonstrated that Snail and related proteins, Worniu and Escargot, have redundant and essential functions in the nervous system. We show that the Snail family of proteins control central nervous system development by regulating genes involved in asymmetry and cell division of neuroblasts. In mutant embryos that have the three genes deleted, the expression of inscuteable is significantly lowered, while the expression of other genes that participate in asymmetric division, including miranda, staufen and prospero, appears normal. The deletion mutants also have much reduced expression of string, suggesting that a key component that drives neuroblast cell division is abnormal. Consistent with the gene expression defects, the mutant embryos lose the asymmetric localization of prospero RNA in neuroblasts and lose the staining of Prospero protein that is normally present in ganglion mother cells. Simultaneous expression of inscuteable and string in the snail family deletion mutant efficiently restores Prospero expression in ganglion mother cells, demonstrating that the two genes are key targets of Snail in neuroblasts. Mutation of the dCtBP co-repressor interaction motifs in the Snail protein leads to reduction of the Snail function in central nervous system. These results suggest that the Snail family of proteins control both asymmetry and cell division of neuroblasts by activating, probably indirectly, the expression of inscuteable and string.</p>
dc.identifier.submissionpathgsbs_sp/59
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages4757-67


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