PGRP-LC and PGRP-LE have essential yet distinct functions in the drosophila immune response to monomeric DAP-type peptidoglycan
Erturk Hasdemir, Deniz
Goldman, William E.
Silverman, Neal S.
UMass Chan AffiliationsDepartment of Medicine, Division of Infectious Disease and Immunology
Graduate School of Biomedical Sciences
Document TypeJournal Article
KeywordsAmino Acid Motifs; Amino Acid Sequence; Animals; Bordetella pertussis; Carrier Proteins; Cell Membrane; Cells, Cultured; Diaminopimelic Acid; Drosophila Proteins; Drosophila melanogaster; Escherichia coli; Gene Expression Regulation; Hemolymph; Intracellular Fluid; Malpighian Tubules; Molecular Sequence Data; Peptide Fragments; Peptidoglycan; RNA Interference; Recombinant Fusion Proteins; Signal Transduction; Transfection; Virulence Factors, Bordetella
Medicine and Health Sciences
MetadataShow full item record
AbstractDrosophila rely entirely on an innate immune response to combat microbial infection. Diaminopimelic acid-containing peptidoglycan, produced by Gram-negative bacteria, is recognized by two receptors, PGRP-LC and PGRP-LE, and activates a homolog of transcription factor NF-kappaB through the Imd signaling pathway. Here we show that full-length PGRP-LE acted as an intracellular receptor for monomeric peptidoglycan, whereas a version of PGRP-LE containing only the PGRP domain functioned extracellularly, like the mammalian CD14 molecule, to enhance PGRP-LC-mediated peptidoglycan recognition on the cell surface. Interaction with the imd signaling protein was not required for PGRP-LC signaling. Instead, PGRP-LC and PGRP-LE signaled through a receptor-interacting protein homotypic interaction motif-like motif. These data demonstrate that like mammals, drosophila use both extracellular and intracellular receptors, which have conserved signaling mechanisms, for innate immune recognition.
SourceNat Immunol. 2006 Jul;7(7):715-23. Epub 2006 Jun 11. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/33938
Related ResourcesLink to article in PubMed