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dc.contributor.authorKislauskis, Edward H.
dc.contributor.authorDobner, Paul R.
dc.date2022-08-11T08:08:59.000
dc.date.accessioned2022-08-23T16:14:50Z
dc.date.available2022-08-23T16:14:50Z
dc.date.issued1990-05-01
dc.date.submitted2008-10-15
dc.identifier.citation<p>Neuron. 1990 May;4(5):783-95.</p>
dc.identifier.issn0896-6273 (Print)
dc.identifier.doi10.1016/0896-6273(90)90205-T
dc.identifier.pmid2344411
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33955
dc.description.abstractThe expression of the gene encoding the neuroendocrine peptides neurotensin (NT) and neuromedin N is strictly dependent on simultaneous exposure to multiple inducers in PC12 pheochromocytoma cells. NT peptide and NT/N mRNA levels are synergistically induced by combinations of NGF, dexamethasone, activators of adenylate cyclase, and lithium ion. We have used transient transfection assays to delineate the rat NT/N gene sequences necessary for this complex regulation. Progressive deletions of the 5' flanking region revealed that sequences between -216 and +56 are sufficient to confer the full spectrum of responses exhibited by the endogenous gene to a reporter gene. Detailed mutational analysis of this region indicates that it is composed of an array of inducible cis-regulatory sequences, including AP-1, cAMP response, and glucocorticoid response elements. Specific mutation of either the AP-1 site or each of two cAMP response elements indicates that they are functionally interdependent. This array of response elements serves to integrate multiple environmental stimuli into a unified transcriptional response.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2344411&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/0896-6273(90)90205-T
dc.subjectAdrenal Gland Neoplasms; Amino Acid Sequence; Animals; Base Sequence; Chromosome Mapping; Fibroblasts; Gene Expression; Genes, Regulator; Molecular Sequence Data; Neurotensin; Peptide Fragments; Phenotype; Pheochromocytoma; Pituitary Gland; Promoter Regions (Genetics); Rats; Tumor Cells, Cultured
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleMutually dependent response elements in the cis-regulatory region of the neurotensin/neuromedin N gene integrate environmental stimuli in PC12 cells
dc.typeJournal Article
dc.source.journaltitleNeuron
dc.source.volume4
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/609
dc.identifier.contextkey651079
html.description.abstract<p>The expression of the gene encoding the neuroendocrine peptides neurotensin (NT) and neuromedin N is strictly dependent on simultaneous exposure to multiple inducers in PC12 pheochromocytoma cells. NT peptide and NT/N mRNA levels are synergistically induced by combinations of NGF, dexamethasone, activators of adenylate cyclase, and lithium ion. We have used transient transfection assays to delineate the rat NT/N gene sequences necessary for this complex regulation. Progressive deletions of the 5' flanking region revealed that sequences between -216 and +56 are sufficient to confer the full spectrum of responses exhibited by the endogenous gene to a reporter gene. Detailed mutational analysis of this region indicates that it is composed of an array of inducible cis-regulatory sequences, including AP-1, cAMP response, and glucocorticoid response elements. Specific mutation of either the AP-1 site or each of two cAMP response elements indicates that they are functionally interdependent. This array of response elements serves to integrate multiple environmental stimuli into a unified transcriptional response.</p>
dc.identifier.submissionpathgsbs_sp/609
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages783-95


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