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dc.contributor.authorKoup, Richard A.
dc.contributor.authorRobinson, James E.
dc.contributor.authorNguyen, Quoc V.
dc.contributor.authorPikora, Cheryl A.
dc.contributor.authorBlais, Bruce
dc.contributor.authorRoskey, Allysen
dc.contributor.authorPanicali, Dennis
dc.contributor.authorSullivan, John L.
dc.date2022-08-11T08:08:59.000
dc.date.accessioned2022-08-23T16:14:54Z
dc.date.available2022-08-23T16:14:54Z
dc.date.issued1991-11-01
dc.date.submitted2008-10-15
dc.identifier.citationAIDS. 1991 Nov;5(11):1309-14.
dc.identifier.issn0269-9370 (Print)
dc.identifier.pmid1722676
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33972
dc.description.abstractWe used a human monoclonal antibody (MAb; 15e) to identify an antibody-dependent cell-mediated cytotoxicity (ADCC) epitope on HIV-1 gp120. 15e has been shown to recognize a conformation-dependent epitope on gp120 which is important in both CD4 binding and neutralizing of HIV-1 infection. 15e binds to gp120 of HIV-1IIIB but not HIV-1RF. Using a standard ADCC assay, 15e was found to mediate ADCC against cells infected with HIV-1IIIB but not HIV-1RF. 15e did not mediate ADCC against cells with recombinant gp120 bound to surface CD4, indicating that 15e does not mediate innocent bystander ADCC against uninfected CD4 cells. To better define the 15e epitope, we performed ADCC against target cells infected with a vaccinia vector which expresses processed HIV-1IIIB gp160 from which the third variable region was deleted (amino acids, 312-328). MAb 15e efficiently mediated ADCC against cells expressing this altered form of gp120, indicating that this region is not contributing to the conformational epitope defined by 15e. 15e defines an important epitope in the human immune response to HIV-1 infection. Antibodies with 15e-like activity may be useful in immunoprophylaxis or immunotherapy of HIV-1 infection.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1722676&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://journals.lww.com/aidsonline/Abstract/1991/11000/Antibody_dependent_cell_mediated_cytotoxicity.4.aspx
dc.subject*Antibodies, Monoclonal; *Antibody-Dependent Cell Cytotoxicity; Binding Sites; Cell Line; Epitopes; Gene Products, env; *HIV Antibodies; HIV Envelope Protein gp120; HIV Envelope Protein gp160; HIV-1; Humans; Protein Precursors
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleAntibody-dependent cell-mediated cytotoxicity directed by a human monoclonal antibody reactive with gp120 of HIV-1
dc.typeJournal Article
dc.source.journaltitleAIDS (London, England)
dc.source.volume5
dc.source.issue11
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/624
dc.identifier.contextkey651094
html.description.abstract<p>We used a human monoclonal antibody (MAb; 15e) to identify an antibody-dependent cell-mediated cytotoxicity (ADCC) epitope on HIV-1 gp120. 15e has been shown to recognize a conformation-dependent epitope on gp120 which is important in both CD4 binding and neutralizing of HIV-1 infection. 15e binds to gp120 of HIV-1IIIB but not HIV-1RF. Using a standard ADCC assay, 15e was found to mediate ADCC against cells infected with HIV-1IIIB but not HIV-1RF. 15e did not mediate ADCC against cells with recombinant gp120 bound to surface CD4, indicating that 15e does not mediate innocent bystander ADCC against uninfected CD4 cells. To better define the 15e epitope, we performed ADCC against target cells infected with a vaccinia vector which expresses processed HIV-1IIIB gp160 from which the third variable region was deleted (amino acids, 312-328). MAb 15e efficiently mediated ADCC against cells expressing this altered form of gp120, indicating that this region is not contributing to the conformational epitope defined by 15e. 15e defines an important epitope in the human immune response to HIV-1 infection. Antibodies with 15e-like activity may be useful in immunoprophylaxis or immunotherapy of HIV-1 infection.</p>
dc.identifier.submissionpathgsbs_sp/624
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentProgram in Immunology and Virology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages1309-14


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