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dc.contributor.authorLai, Sen-Lin
dc.contributor.authorLee, Tzumin
dc.date2022-08-11T08:09:00.000
dc.date.accessioned2022-08-23T16:14:57Z
dc.date.available2022-08-23T16:14:57Z
dc.date.issued2006-05-01
dc.date.submitted2008-10-15
dc.identifier.citationNat Neurosci. 2006 May;9(5):703-9. Epub 2006 Apr 2. <a href="http://dx.doi.org/10.1038/nn1681 ">Link to article on publisher's site</a>
dc.identifier.issn1097-6256 (Print)
dc.identifier.doi10.1038/nn1681
dc.identifier.pmid16582903
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33983
dc.description.abstractMARCM (mosaic analysis with a repressible cell marker) involves specific labeling of GAL80-minus and GAL4-positive homozygous cells in otherwise heterozygous tissues. Here we demonstrate how the concurrent use of two independent binary transcriptional systems may facilitate complex MARCM studies in the Drosophila nervous system. By fusing LexA with the VP16 acidic activation domain (VP16) or the GAL4 activation domain (GAD), we obtained both GAL80-insensitive and GAL80-suppressible transcriptional factors. LexA::VP16 can mediate MARCM-independent binary transgene induction in mosaic organisms. The incorporation of LexA::GAD into MARCM, which we call dual-expression-control MARCM, permits the induction of distinct transgenes in different patterns among GAL80-minus cells in mosaic tissues. Lineage analysis with dual-expression-control MARCM suggested the presence of neuroglioblasts in the developing optic lobes but did not indicate the production of glia by postembryonic mushroom body neuronal precursors. In addition, dual-expression-control MARCM with a ubiquitous LexA::GAD driver revealed many unidentified cells in the GAL4-GH146-positive projection neuron lineages.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16582903&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1038/nn1681
dc.subjectAnimals; Animals, Genetically Modified; Brain; Drosophila; Drosophila Proteins; *Gene Expression Regulation, Developmental; Immunohistochemistry; Models, Molecular; *Mosaicism; Neurons; Repressor Proteins; Trans-Activation (Genetics); Transcription Factors
dc.subjectNeuroscience and Neurobiology
dc.titleGenetic mosaic with dual binary transcriptional systems in Drosophila
dc.typeJournal Article
dc.source.journaltitleNature neuroscience
dc.source.volume9
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/636
dc.identifier.contextkey651106
html.description.abstract<p>MARCM (mosaic analysis with a repressible cell marker) involves specific labeling of GAL80-minus and GAL4-positive homozygous cells in otherwise heterozygous tissues. Here we demonstrate how the concurrent use of two independent binary transcriptional systems may facilitate complex MARCM studies in the Drosophila nervous system. By fusing LexA with the VP16 acidic activation domain (VP16) or the GAL4 activation domain (GAD), we obtained both GAL80-insensitive and GAL80-suppressible transcriptional factors. LexA::VP16 can mediate MARCM-independent binary transgene induction in mosaic organisms. The incorporation of LexA::GAD into MARCM, which we call dual-expression-control MARCM, permits the induction of distinct transgenes in different patterns among GAL80-minus cells in mosaic tissues. Lineage analysis with dual-expression-control MARCM suggested the presence of neuroglioblasts in the developing optic lobes but did not indicate the production of glia by postembryonic mushroom body neuronal precursors. In addition, dual-expression-control MARCM with a ubiquitous LexA::GAD driver revealed many unidentified cells in the GAL4-GH146-positive projection neuron lineages.</p>
dc.identifier.submissionpathgsbs_sp/636
dc.contributor.departmentGraduate School of Biomedical Sciences, Neuroscience Program
dc.contributor.departmentLee Lab
dc.contributor.departmentNeurobiology
dc.source.pages703-9
dc.contributor.studentSen-Lin Lai
dc.description.thesisprogramNeuroscience


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