Repressor elements in the coding region of the human histone H4 gene interact with the transcription factor CDP/cut
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Document Type
Journal ArticlePublication Date
1999-01-05Keywords
Base Sequence; Binding Sites; Cell Cycle; Cell Division; DNA; Genes; Histones; Homeodomain Proteins; Humans; Nuclear Proteins; Regulatory Sequences, Nucleic Acid; Repressor Proteins; Sequence Homology, Nucleic Acid; Transcription Factors; Transcription, GeneticLife Sciences
Medicine and Health Sciences
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The coding region of the human histone H4 gene FO108 undergoes dynamic changes in chromatin structure that correlate with modifications in gene expression. Such structural alterations generally reflect transcription factor interactions with gene regulatory sequences. To test for regulatory elements within the coding region, we performed transient transfection experiments in HeLa cells using constructs with histone H4 sequences fused upstream of a heterologous thymidine kinase promoter and CAT reporter gene. H4 gene sequences from -10 to +210 repressed transcription 4.8-fold. Further deletion and mutational analysis delineated three repressor elements within this region. Using oligonucleotide competition analysis and specific antibody recognition in electrophoretic mobility shift assays, as well as methylation interference and DNase I footprinting analyses, we have identified the CCAAT displacement protein (CDP/cut) as the factor that interacts with these three repressor elements. CDP/cut binding to these repressor sites is proliferation-specific and cell-cycle-regulated, increasing in mid to late S phase. Our results indicate that the proximal 200 nucleotides of the histone H4-coding region contain transcriptional regulatory elements that may contribute to cell-cycle control of histone gene expression by interacting with repressor complexes containing CDP/cut homeodomain transcription factors.Source
Gene. 1998 Oct 23;221(2):267-77.
DOI
10.1016/S0378-1119(98)00415-6Permanent Link to this Item
http://hdl.handle.net/20.500.14038/33990PubMed ID
9874597Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/S0378-1119(98)00415-6