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dc.contributor.authorLast, Thomas J.
dc.contributor.authorBirnbaum, Mark J.
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorStein, Gary S.
dc.contributor.authorStein, Janet L.
dc.date2022-08-11T08:09:00.000
dc.date.accessioned2022-08-23T16:14:58Z
dc.date.available2022-08-23T16:14:58Z
dc.date.issued1999-01-05
dc.date.submitted2008-10-15
dc.identifier.citation<p>Gene. 1998 Oct 23;221(2):267-77.</p>
dc.identifier.issn0378-1119 (Print)
dc.identifier.doi10.1016/S0378-1119(98)00415-6
dc.identifier.pmid9874597
dc.identifier.urihttp://hdl.handle.net/20.500.14038/33990
dc.description.abstractThe coding region of the human histone H4 gene FO108 undergoes dynamic changes in chromatin structure that correlate with modifications in gene expression. Such structural alterations generally reflect transcription factor interactions with gene regulatory sequences. To test for regulatory elements within the coding region, we performed transient transfection experiments in HeLa cells using constructs with histone H4 sequences fused upstream of a heterologous thymidine kinase promoter and CAT reporter gene. H4 gene sequences from -10 to +210 repressed transcription 4.8-fold. Further deletion and mutational analysis delineated three repressor elements within this region. Using oligonucleotide competition analysis and specific antibody recognition in electrophoretic mobility shift assays, as well as methylation interference and DNase I footprinting analyses, we have identified the CCAAT displacement protein (CDP/cut) as the factor that interacts with these three repressor elements. CDP/cut binding to these repressor sites is proliferation-specific and cell-cycle-regulated, increasing in mid to late S phase. Our results indicate that the proximal 200 nucleotides of the histone H4-coding region contain transcriptional regulatory elements that may contribute to cell-cycle control of histone gene expression by interacting with repressor complexes containing CDP/cut homeodomain transcription factors.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9874597&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/S0378-1119(98)00415-6
dc.subjectBase Sequence; Binding Sites; Cell Cycle; Cell Division; DNA; Genes; Histones; Homeodomain Proteins; Humans; Nuclear Proteins; Regulatory Sequences, Nucleic Acid; Repressor Proteins; Sequence Homology, Nucleic Acid; Transcription Factors; Transcription, Genetic
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleRepressor elements in the coding region of the human histone H4 gene interact with the transcription factor CDP/cut
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume221
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/643
dc.identifier.contextkey651113
html.description.abstract<p>The coding region of the human histone H4 gene FO108 undergoes dynamic changes in chromatin structure that correlate with modifications in gene expression. Such structural alterations generally reflect transcription factor interactions with gene regulatory sequences. To test for regulatory elements within the coding region, we performed transient transfection experiments in HeLa cells using constructs with histone H4 sequences fused upstream of a heterologous thymidine kinase promoter and CAT reporter gene. H4 gene sequences from -10 to +210 repressed transcription 4.8-fold. Further deletion and mutational analysis delineated three repressor elements within this region. Using oligonucleotide competition analysis and specific antibody recognition in electrophoretic mobility shift assays, as well as methylation interference and DNase I footprinting analyses, we have identified the CCAAT displacement protein (CDP/cut) as the factor that interacts with these three repressor elements. CDP/cut binding to these repressor sites is proliferation-specific and cell-cycle-regulated, increasing in mid to late S phase. Our results indicate that the proximal 200 nucleotides of the histone H4-coding region contain transcriptional regulatory elements that may contribute to cell-cycle control of histone gene expression by interacting with repressor complexes containing CDP/cut homeodomain transcription factors.</p>
dc.identifier.submissionpathgsbs_sp/643
dc.contributor.departmentDepartment of Cell Biology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages267-77


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