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dc.contributor.authorLee, Kai-Fai
dc.contributor.authorLau, Kin-Mang
dc.contributor.authorHo, Shuk-Mei
dc.date2022-08-11T08:09:00.000
dc.date.accessioned2022-08-23T16:15:01Z
dc.date.available2022-08-23T16:15:01Z
dc.date.issued2000-01-06
dc.date.submitted2008-10-15
dc.identifier.citationToxicol Appl Pharmacol. 1999 Dec 15;161(3):294-301. <a href="http://dx.doi.org/10.1006/taap.1999.8821 ">Link to article on publisher's site</a>
dc.identifier.issn0041-008X (Print)
dc.identifier.doi10.1006/taap.1999.8821
dc.identifier.pmid10620487
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34000
dc.description.abstractTwo trans-acting hammerhead ribozymes (Rzs), Rz1-2 and Rz4-9, were designed and shown to be effective in cleaving rat or mouse metallothenein (MT)-I and MT-II mRNA, respectively, at position +147/148, in a sequence-specific manner. The catalytic efficiency for Rz1-2 on rat MT-I was found to be 678 M(-1)s(-1) and that for Rz4-9 on rat MT-II cRNA was found to be 372 M(-1)s(-1). An expression vector, pRz(4-9/1-2), containing both Rzs linked in tandem, was constructed and used to transfect NbE-1 cells, an immortalized ventral prostate epithelial cell line. Two stable-transfected lines, NbE-1(Rz2) and NbE-1(Rz3), expressing substantial levels of (Rz1-2/Rz4-9) and minimal levels of MT-I and MT-II transcripts were found to exhibit increased sensitivity to cadmium (Cd)-induced cytotoxicity compared to the parent line. This article is the first to report successful degradation of rodent MT mRNA in vitro and in cellulo by hammerhead Rzs.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10620487&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1006/taap.1999.8821
dc.subjectAnimals; Base Sequence; Cadmium; Cell Line, Transformed; Cell Survival; Clone Cells; DNA Primers; Epithelial Cells; Male; Metallothionein; Mice; Molecular Sequence Data; Nucleic Acid Conformation; Prostate; RNA, Catalytic; RNA, Messenger; Rats; Transcription, Genetic; Transfection
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleGeneration and characterization of hammerhead ribozymes targeting rodent metallothionein-I and -II ribonucleic acid
dc.typeJournal Article
dc.source.journaltitleToxicology and applied pharmacology
dc.source.volume161
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/653
dc.identifier.contextkey651123
html.description.abstract<p>Two trans-acting hammerhead ribozymes (Rzs), Rz1-2 and Rz4-9, were designed and shown to be effective in cleaving rat or mouse metallothenein (MT)-I and MT-II mRNA, respectively, at position +147/148, in a sequence-specific manner. The catalytic efficiency for Rz1-2 on rat MT-I was found to be 678 M(-1)s(-1) and that for Rz4-9 on rat MT-II cRNA was found to be 372 M(-1)s(-1). An expression vector, pRz(4-9/1-2), containing both Rzs linked in tandem, was constructed and used to transfect NbE-1 cells, an immortalized ventral prostate epithelial cell line. Two stable-transfected lines, NbE-1(Rz2) and NbE-1(Rz3), expressing substantial levels of (Rz1-2/Rz4-9) and minimal levels of MT-I and MT-II transcripts were found to exhibit increased sensitivity to cadmium (Cd)-induced cytotoxicity compared to the parent line. This article is the first to report successful degradation of rodent MT mRNA in vitro and in cellulo by hammerhead Rzs.</p>
dc.identifier.submissionpathgsbs_sp/653
dc.contributor.departmentDepartment of Surgery
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages294-301


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