Cloning and intracellular localization of the U2 small nuclear ribonucleoprotein auxiliary factor small subunit
UMass Chan Affiliations
Program in Gene Function and ExpressionProgram in Molecular Medicine
Department of Biochemistry and Molecular Pharmacology
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
1992-09-15Keywords
Amino Acid Sequence; Base Sequence; Cell Nucleus; Gene Expression; Hela Cells; Humans; Macromolecular Substances; Molecular Sequence Data; Nuclear Proteins; RNA Splicing; RNA, Messenger; RNA, Small Nuclear; Ribonucleoproteins; Ribonucleoproteins, Small NuclearLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
U2 small nuclear ribonucleoprotein auxiliary factor (U2AF), an essential mammalian splicing factor, is composed of two subunits: a 65-kDa protein (U2AF65), which binds the pre-mRNA polypyrimidine tract and is required for in vitro splicing, and an associated 35-kDa protein (U2AF35). Here we report the isolation of a cDNA encoding U2AF35. U2AF35 contains sequence motifs found in several mammalian pre-mRNA splicing factors. We show directly that U2AF65 and U2AF35 interact with each other and delineate the regions of both proteins that mediate this interaction. Using anti-peptide antibodies against U2AF35, we show that the protein has the intracellular distribution characteristic of U2AF65. Both U2AF65 and U2AF35 are concentrated in a small number of nuclear foci corresponding to coiled bodies, subnuclear organelles first identified by light microscopy in 1903.Source
Proc Natl Acad Sci U S A. 1992 Sep 15;89(18):8769-73.
DOI
10.1073/pnas.89.18.8769Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34003PubMed ID
1388271Related Resources
ae974a485f413a2113503eed53cd6c53
10.1073/pnas.89.18.8769