DNA translocation and loop formation mechanism of chromatin remodeling by SWI/SNF and RSC
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Authors
Zhang, YongliSmith, Corey Lewis
Saha, Anjanabha
Grill, Stephan W.
Mihardja, Shirley
Smith, Steven B.
Cairns, Bradley R.
Peterson, Craig L.
Bustamante, Carlos
UMass Chan Affiliations
Program in Molecular MedicineProgram in Gene Function and Expression
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2006-12-26Keywords
Adenosine Triphosphate; Animals; Chickens; *Chromatin Assembly and Disassembly; Chromosomal Proteins, Non-Histone; DNA; DNA-Binding Proteins; Models, Biological; Models, Molecular; *Nucleic Acid Conformation; Nucleosomes; Optical Tweezers; Saccharomyces cerevisiae Proteins; Stress, Mechanical; Tandem Repeat Sequences; Transcription FactorsLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
ATP-dependent chromatin-remodeling complexes (remodelers) modulate gene transcription by regulating the accessibility of highly packaged genomic DNA. However, the molecular mechanisms involved at the nucleosomal level in this process remain controversial. Here, we monitor the real-time activity of single ySWI/SNF or RSC complexes on single, stretched nucleosomal templates under tensions above 1 pN forces. We find that these remodelers can translocate along DNA at rates of approximately 13 bp/s and generate forces up to approximately 12 pN, producing DNA loops of a broad range of sizes (20-1200 bp, average approximately 100 bp) in a nucleosome-dependent manner. This nucleosome-specific activity differs significantly from that on bare DNA observed under low tensions and suggests a nucleosome-remodeling mechanism through intranucleosomal DNA loop formation. Such loop formation may provide a molecular basis for the biological functions of remodelers.Source
Mol Cell. 2006 Nov 17;24(4):559-68. Link to article on publisher's siteDOI
10.1016/j.molcel.2006.10.025Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34005PubMed ID
17188033Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.molcel.2006.10.025