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    Mitotic partitioning and selective reorganization of tissue-specific transcription factors in progeny cells

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    Authors
    Zaidi, Sayyed K.
    Young, Daniel W.
    Pockwinse, Shirwin M.
    Javed, Amjad
    Lian, Jane B.
    Stein, Janet L.
    Van Wijnen, Andre J.
    Stein, Gary S.
    UMass Chan Affiliations
    Graduate School of Biomedical Sciences
    Department of Cell Biology
    Document Type
    Journal Article
    Publication Date
    2003-12-06
    Keywords
    Animals; Cell Division; Cell Line, Tumor; Cell Nucleus; Chromatin; DNA; G2 Phase; Histones; Humans; Image Processing, Computer-Assisted; Jurkat Cells; Microscopy, Fluorescence; *Mitosis; Nuclear Proteins; Phenotype; Rats; *Ribonucleoproteins; Telophase; Time Factors; Transcription Factors
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    http://dx.doi.org/10.1073/pnas.2533076100
    Abstract
    Postmitotic gene expression requires restoration of nuclear organization and assembly of regulatory complexes. The hematopoietic and osteogenic Runx (Cbfa/AML) transcription factors are punctately organized in the interphase nucleus and provide a model for understanding the subnuclear organization of tissue-specific regulatory proteins after mitosis. Here we have used quantitative in situ immunofluorescence microscopy and quantitative image analysis to show that Runx factors undergo progressive changes in cellular localization during mitosis while retaining a punctate distribution. In comparison, the acetyl transferase p300 and acetylated histone H4 remain localized with DNA throughout mitosis while the RNA processing factor SC35 is excluded from mitotic chromatin. Subnuclear organization of Runx foci is completely restored in telophase, and Runx proteins are equally partitioned into progeny nuclei. In contrast, subnuclear organization of SC35 is restored subsequent to telophase. Our results show a sequential reorganization of Runx and its coregulatory proteins that precedes restoration of RNA processing speckles. Thus, mitotic partitioning and spatiotemporal reorganization of regulatory proteins together render progeny cells equivalently competent to support phenotypic gene expression.
    Source
    Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14852-7. Epub 2003 Dec 1. Link to article on publisher's site
    DOI
    10.1073/pnas.2533076100
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34067
    PubMed ID
    14657346
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1073/pnas.2533076100
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    Morningside Graduate School of Biomedical Sciences Scholarly Publications

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