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    Runt homology domain proteins in osteoblast differentiation: AML3/CBFA1 is a major component of a bone-specific complex

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    Authors
    Banerjee, Chaitali
    McCabe, Laura R.
    Choi, Je-Yong
    Hiebert, Scott W.
    Stein, Janet L.
    Stein, Gary S.
    Lian, Jane B.
    UMass Chan Affiliations
    Department of Cell Biology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    1997-07-01
    Keywords
    Animals; Cell Differentiation; Cell Line; Core Binding Factor Alpha 1 Subunit; DNA-Binding Proteins; Drosophila Proteins; Gene Expression Regulation, Developmental; *Neoplasm Proteins; Nuclear Proteins; Osteoblasts; Osteocalcin; RNA, Messenger; Rats; Trans-Activation (Genetics); Transcription Factors
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://doi.org/10.1002/(SICI)1097-4644(19970701)66:1<1::AID-JCB1>3.0.CO;2-V
    Abstract
    The AML/CBFA family of runt homology domain (rhd) transcription factors regulates expression of mammalian genes of the hematopoietic lineage. AML1, AML2 and AML3 are the three AML genes identified to date which influence myeloid cell growth and differentiation. Recently AML-related proteins were identified in an osteoblast-specific promoter binding complex that functionally modulates bone-restricted transcription of the osteocalcin gene. In the present study we demonstrate that in primary rat osteoblasts AML-3 is the AML family member present in the osteoblast-specific complex. Antibody specific for AML-3 completely supershifts this complex, in contrast to antibodies with specificity for AML-1 or AML-2, AML-3 is present as a single 5.4 kb transcript in bone tissues. To establish the functional involvement of AML factors in osteoblast differentiation, we pursued antisense strategies to alter expression of rhd genes. Treatment of osteoblast cultures with rhd antisense oligonucleotides significantly decreased three parameters which are linked to differentiation of normal diploid osteoblasts: the representation of alkaline phosphatase-positive cells, osteocalcin production, and the formation of mineralized nodules. Our findings indicate that AML-3 is a key transcription factor in bone cells and that the activity of rhd proteins is required for completion of osteoblast differentiation.
    Source

    J Cell Biochem. 1997 Jul 1;66(1):1-8.

    DOI
    10.1002/(SICI)1097-4644(19970701)66:1<1::AID-JCB1>3.0.CO;2-V
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34078
    PubMed ID
    9215522
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    ae974a485f413a2113503eed53cd6c53
    10.1002/(SICI)1097-4644(19970701)66:1<1::AID-JCB1>3.0.CO;2-V
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