Higher level organization of individual gene transcription and RNA splicing
Document Type
Journal ArticlePublication Date
1993-02-26Keywords
Animals; Cell Nucleus; Fibronectins; Gene Expression; Introns; Microscopy, Fluorescence; Neurotensin; PC12 Cells; Poly A; *RNA Processing, Post-Transcriptional; RNA Splicing; RNA, Messenger; Rats; Spliceosomes; *Transcription, GeneticCell Biology
Life Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Visualization of fibronectin and neurotensin messenger RNAs within mammalian interphase nuclei was achieved by fluorescence hybridization with genomic, complementary DNA, and intron-specific probes. Unspliced transcripts accumulated in one or two sites per nucleus. Fibronectin RNA frequently accumulated in elongated tracks that overlapped and extended well beyond the site of transcription. Splicing appears to occur directly within this RNA track, as evidenced by an unambiguous spatial separation of intron-containing and spliced transcripts. Excised introns for neurotensin RNA appear free to diffuse. The transcription and processing site of the fibronectin gene localized to the nuclear interior and was associated with larger transcript domains in over 88 percent of the cells. These results support a view of nuclear function closely integrated with structure.Source
Science. 1993 Feb 26;259(5099):1326-30.
DOI
10.1126/science.8446901Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34080PubMed ID
8446901Related Resources
ae974a485f413a2113503eed53cd6c53
10.1126/science.8446901