Simian immunodeficiency virus-specific cytotoxic T-lymphocyte induction through DNA vaccination of rhesus monkeys
Robinson, Harriet L.
Mullins, James I.
Letvin, Norman L.
UMass Chan AffiliationsDepartment of Pathology
Department of Medicine
Graduate School of Biomedical Sciences
KeywordsAnimals; DNA, Viral; Gene Products, env; Histocompatibility Antigens Class I; Macaca mulatta; Plasmids; Retroviridae Proteins; Simian immunodeficiency virus; T-Lymphocytes, Cytotoxic; Tumor Cells, Cultured; Vaccines, Synthetic; Viral Envelope Proteins; Viral Vaccines
Medicine and Health Sciences
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AbstractIn view of the growing evidence that virus-specific cytotoxic T lymphocytes (CTL) play an important role in containing the early spread of human immunodeficiency virus type 1 (HIV-1) in infected individuals, novel vaccine strategies capable of eliciting HIV-1-specific CTL are being pursued in attempts to create an effective AIDS vaccine. We have used the simian immunodeficiency virus of macaques (SIVmac)/rhesus monkey model to explore the induction of AIDS virus-specific CTL responses by DNA vaccination. We found that the inoculation of rhesus monkeys with plasmid DNA encoding SIVmac Env and Gag elicited a persisting SIVmac-specific memory CTL response. These CTL were CD8+ and major histocompatibility complex class I restricted. These studies provide evidence for the potential utility of DNA inoculation as an approach to an HIV-1 vaccine.
J Virol. 1996 Jan;70(1):678-81.