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dc.contributor.authorLindenmuth, Danielle M.
dc.contributor.authorVan Wijnen, Andre J.
dc.contributor.authorPenman, Sheldon
dc.contributor.authorStein, Janet L.
dc.contributor.authorStein, Gary S.
dc.contributor.authorLian, Jane B.
dc.date2022-08-11T08:09:01.000
dc.date.accessioned2022-08-23T16:15:30Z
dc.date.available2022-08-23T16:15:30Z
dc.date.issued1998-05-15
dc.date.submitted2008-11-05
dc.identifier.citationJ Cell Biochem. 1998 Jun 1;69(3):291-303.
dc.identifier.issn0730-2312 (Print)
dc.identifier.pmid9581868
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34103
dc.description.abstractNuclear matrix protein (NMP) composition of osteoblasts shows distinct two-dimensional gel electrophoretic profiles of labeled proteins as a function of stages of cellular differentiation. Because NMPs are involved in the control of gene expression, we examined modifications in the representation of NMPs induced by TGF-beta1 treatment of osteoblasts to gain insight into the effects of TGF-beta on development of the osteoblast phenotype. Exposure of proliferating fetal rat calvarial derived primary cells in culture to TGF-beta1 for 48 h (day 4-6) modifies osteoblast cell morphology and proliferation and blocks subsequent formation of mineralized nodules. Nuclear matrix protein profiles were very similar between control and TGF-beta-treated cultures until day 14, but subsequently differences in nuclear matrix proteins were apparent in TGF-beta-treated cultures. These findings support the concept that TGF-beta1 modifies the final stage of osteoblast mineralization and alters the composition of the osteoblast nuclear matrix as reflected by selective and TGF-beta-dependent modifications in the levels of specific nuclear matrix proteins. The specific changes induced by TGF-beta in nuclear matrix associated proteins may reflect specialized mechanisms by which TGF-beta signalling mediates the alterations in cell organization and nodule formation and/or the consequential block in extracellular mineralization.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9581868&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/(SICI)1097-4644(19980601)69:3<291::AID-JCB7>3.0.CO;2-M
dc.subjectAnimals; Antigens, Nuclear; *Cell Differentiation; Electrophoresis, Gel, Two-Dimensional; Extracellular Matrix; Nuclear Proteins; Osteoblasts; Rats; Rats, Sprague-Dawley; Transforming Growth Factor beta
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleTGF-beta1 modifications in nuclear matrix proteins of osteoblasts during differentiation
dc.typeJournal Article
dc.source.journaltitleJournal of cellular biochemistry
dc.source.volume69
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/767
dc.identifier.contextkey661876
html.description.abstract<p>Nuclear matrix protein (NMP) composition of osteoblasts shows distinct two-dimensional gel electrophoretic profiles of labeled proteins as a function of stages of cellular differentiation. Because NMPs are involved in the control of gene expression, we examined modifications in the representation of NMPs induced by TGF-beta1 treatment of osteoblasts to gain insight into the effects of TGF-beta on development of the osteoblast phenotype. Exposure of proliferating fetal rat calvarial derived primary cells in culture to TGF-beta1 for 48 h (day 4-6) modifies osteoblast cell morphology and proliferation and blocks subsequent formation of mineralized nodules. Nuclear matrix protein profiles were very similar between control and TGF-beta-treated cultures until day 14, but subsequently differences in nuclear matrix proteins were apparent in TGF-beta-treated cultures. These findings support the concept that TGF-beta1 modifies the final stage of osteoblast mineralization and alters the composition of the osteoblast nuclear matrix as reflected by selective and TGF-beta-dependent modifications in the levels of specific nuclear matrix proteins. The specific changes induced by TGF-beta in nuclear matrix associated proteins may reflect specialized mechanisms by which TGF-beta signalling mediates the alterations in cell organization and nodule formation and/or the consequential block in extracellular mineralization.</p>
dc.identifier.submissionpathgsbs_sp/767
dc.contributor.departmentDepartment of Cell Biology and Cancer Center
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages291-303


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