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dc.contributor.authorLuzuriaga, Katherine
dc.contributor.authorKoup, Richard A.
dc.contributor.authorPikora, Cheryl A.
dc.contributor.authorBrettler, Doreen B.
dc.contributor.authorSullivan, John L.
dc.date2022-08-11T08:09:01.000
dc.date.accessioned2022-08-23T16:15:37Z
dc.date.available2022-08-23T16:15:37Z
dc.date.issued1991-08-01
dc.date.submitted2008-11-05
dc.identifier.citation<p>J Pediatr. 1991 Aug;119(2):230-6.</p>
dc.identifier.issn0022-3476 (Print)
dc.identifier.doi10.1016/S0022-3476(05)80732-2
dc.identifier.pmid1907319
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34132
dc.description.abstractCytotoxic T lymphocyte (CTL) responses to human immunodeficiency virus type 1 (HIV-1) gag proteins were studied prospectively in 17 children (12 infected) born of mothers with HIV-1 seropositivity and in five pediatric patients with hemophilia infected by transfusion of HIV-1-contaminated factor VIII concentrate. B lymphoblastoid cells infected with vaccinia virus vectors expressing HIV-1 gag gene products were combined with autologous peripheral blood mononuclear cells to detect circulating CTLs. Effector cells were defined by monoclonal antibody-mediated, complement-dependent cytolysis. Circulating HIV-1 gag-specific cytotoxic responses were detectable in 4 of 5 HIV-1-infected pediatric hemophilic patients, and were similar in magnitude to those previously described in adults. In contrast, circulating HIV-1 gag-specific cytolysis was detectible in only 3 of 12 vertically infected children. Depletion data revealed that the majority of detectible gag-specific cytolysis was CD8 T cell-mediated. No apparent relationships between CD4 T cell counts, CD8 T cells counts, or serum p24 antigen levels and CTL responses were seen. Deficient CTL development may, in part, explain the more rapid onset of symptomatic disease following vertical HIV infection.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1907319&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/S0022-3476(05)80732-2
dc.subjectAcquired Immunodeficiency; Syndrome; Adolescent; Antigens, CD; Antigens, CD4; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Child; Child, Preschool; Cytotoxicity Tests, Immunologic; Gene Products, gag; HIV Antigens; HIV Core Protein p24; HIV Infections; HIV-1; Hemophilia A; Humans; Infant; T-Lymphocytes, Cytotoxic; Viral Core Proteins; Virus Cultivation
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleDeficient human immunodeficiency virus type 1-specific cytotoxic T cell responses in vertically infected children
dc.typeJournal Article
dc.source.journaltitleThe Journal of pediatrics
dc.source.volume119
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/794
dc.identifier.contextkey661904
html.description.abstract<p>Cytotoxic T lymphocyte (CTL) responses to human immunodeficiency virus type 1 (HIV-1) gag proteins were studied prospectively in 17 children (12 infected) born of mothers with HIV-1 seropositivity and in five pediatric patients with hemophilia infected by transfusion of HIV-1-contaminated factor VIII concentrate. B lymphoblastoid cells infected with vaccinia virus vectors expressing HIV-1 gag gene products were combined with autologous peripheral blood mononuclear cells to detect circulating CTLs. Effector cells were defined by monoclonal antibody-mediated, complement-dependent cytolysis. Circulating HIV-1 gag-specific cytotoxic responses were detectable in 4 of 5 HIV-1-infected pediatric hemophilic patients, and were similar in magnitude to those previously described in adults. In contrast, circulating HIV-1 gag-specific cytolysis was detectible in only 3 of 12 vertically infected children. Depletion data revealed that the majority of detectible gag-specific cytolysis was CD8 T cell-mediated. No apparent relationships between CD4 T cell counts, CD8 T cells counts, or serum p24 antigen levels and CTL responses were seen. Deficient CTL development may, in part, explain the more rapid onset of symptomatic disease following vertical HIV infection.</p>
dc.identifier.submissionpathgsbs_sp/794
dc.contributor.departmentDepartment of Medicine, Division of Hematology and Oncology
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentProgram in Immunology and Virology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages230-6


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