Deficient human immunodeficiency virus type 1-specific cytotoxic T cell responses in vertically infected children
dc.contributor.author | Luzuriaga, Katherine | |
dc.contributor.author | Koup, Richard A. | |
dc.contributor.author | Pikora, Cheryl A. | |
dc.contributor.author | Brettler, Doreen B. | |
dc.contributor.author | Sullivan, John L. | |
dc.date | 2022-08-11T08:09:01.000 | |
dc.date.accessioned | 2022-08-23T16:15:37Z | |
dc.date.available | 2022-08-23T16:15:37Z | |
dc.date.issued | 1991-08-01 | |
dc.date.submitted | 2008-11-05 | |
dc.identifier.citation | <p>J Pediatr. 1991 Aug;119(2):230-6.</p> | |
dc.identifier.issn | 0022-3476 (Print) | |
dc.identifier.doi | 10.1016/S0022-3476(05)80732-2 | |
dc.identifier.pmid | 1907319 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/34132 | |
dc.description.abstract | Cytotoxic T lymphocyte (CTL) responses to human immunodeficiency virus type 1 (HIV-1) gag proteins were studied prospectively in 17 children (12 infected) born of mothers with HIV-1 seropositivity and in five pediatric patients with hemophilia infected by transfusion of HIV-1-contaminated factor VIII concentrate. B lymphoblastoid cells infected with vaccinia virus vectors expressing HIV-1 gag gene products were combined with autologous peripheral blood mononuclear cells to detect circulating CTLs. Effector cells were defined by monoclonal antibody-mediated, complement-dependent cytolysis. Circulating HIV-1 gag-specific cytotoxic responses were detectable in 4 of 5 HIV-1-infected pediatric hemophilic patients, and were similar in magnitude to those previously described in adults. In contrast, circulating HIV-1 gag-specific cytolysis was detectible in only 3 of 12 vertically infected children. Depletion data revealed that the majority of detectible gag-specific cytolysis was CD8 T cell-mediated. No apparent relationships between CD4 T cell counts, CD8 T cells counts, or serum p24 antigen levels and CTL responses were seen. Deficient CTL development may, in part, explain the more rapid onset of symptomatic disease following vertical HIV infection. | |
dc.language.iso | en_US | |
dc.relation | <p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=1907319&dopt=Abstract">Link to article in PubMed</a></p> | |
dc.relation.url | https://doi.org/10.1016/S0022-3476(05)80732-2 | |
dc.subject | Acquired Immunodeficiency; Syndrome; Adolescent; Antigens, CD; Antigens, CD4; Antigens, CD8; Antigens, Differentiation, T-Lymphocyte; Child; Child, Preschool; Cytotoxicity Tests, Immunologic; Gene Products, gag; HIV Antigens; HIV Core Protein p24; HIV Infections; HIV-1; Hemophilia A; Humans; Infant; T-Lymphocytes, Cytotoxic; Viral Core Proteins; Virus Cultivation | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | Deficient human immunodeficiency virus type 1-specific cytotoxic T cell responses in vertically infected children | |
dc.type | Journal Article | |
dc.source.journaltitle | The Journal of pediatrics | |
dc.source.volume | 119 | |
dc.source.issue | 2 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/794 | |
dc.identifier.contextkey | 661904 | |
html.description.abstract | <p>Cytotoxic T lymphocyte (CTL) responses to human immunodeficiency virus type 1 (HIV-1) gag proteins were studied prospectively in 17 children (12 infected) born of mothers with HIV-1 seropositivity and in five pediatric patients with hemophilia infected by transfusion of HIV-1-contaminated factor VIII concentrate. B lymphoblastoid cells infected with vaccinia virus vectors expressing HIV-1 gag gene products were combined with autologous peripheral blood mononuclear cells to detect circulating CTLs. Effector cells were defined by monoclonal antibody-mediated, complement-dependent cytolysis. Circulating HIV-1 gag-specific cytotoxic responses were detectable in 4 of 5 HIV-1-infected pediatric hemophilic patients, and were similar in magnitude to those previously described in adults. In contrast, circulating HIV-1 gag-specific cytolysis was detectible in only 3 of 12 vertically infected children. Depletion data revealed that the majority of detectible gag-specific cytolysis was CD8 T cell-mediated. No apparent relationships between CD4 T cell counts, CD8 T cells counts, or serum p24 antigen levels and CTL responses were seen. Deficient CTL development may, in part, explain the more rapid onset of symptomatic disease following vertical HIV infection.</p> | |
dc.identifier.submissionpath | gsbs_sp/794 | |
dc.contributor.department | Department of Medicine, Division of Hematology and Oncology | |
dc.contributor.department | Department of Pediatrics | |
dc.contributor.department | Program in Immunology and Virology | |
dc.contributor.department | Graduate School of Biomedical Sciences | |
dc.source.pages | 230-6 |