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dc.contributor.authorMelanson, Vanessa Rose
dc.contributor.authorIorio, Ronald M.
dc.date2022-08-11T08:09:01.000
dc.date.accessioned2022-08-23T16:15:51Z
dc.date.available2022-08-23T16:15:51Z
dc.date.issued2005-12-28
dc.date.submitted2008-11-21
dc.identifier.citationJ Virol. 2006 Jan;80(2):623-33. <a href="http://dx.doi.org/10.1128/JVI.80.2.623-633.2006 ">Link to article on publisher's site</a>
dc.identifier.issn0022-538X (Print)
dc.identifier.doi10.1128/JVI.80.2.623-633.2006
dc.identifier.pmid16378965
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34189
dc.description.abstractMost paramyxovirus fusion (F) proteins require the coexpression of the homologous attachment (HN) protein to promote membrane fusion, consistent with the existence of a virus-specific interaction between the two proteins. Analysis of the fusion activities of chimeric HN proteins indicates that the stalk region of the HN spike determines its F protein specificity, and analysis of a panel of site-directed mutants indicates that the F-interactive site resides in this region. Here, we use the addition of oligosaccharides to further explore the role of the HN stalk in the interaction with F. N-glycans were individually added at several positions in the stalk to determine their effects on the activities of HN, as well as its structure. N-glycan addition at positions 69 and 77 in the stalk specifically blocks fusion and the HN-F interaction without affecting either HN structure or its other activities. N-glycans added at other positions in the stalk modulate activities that reside in the globular head of HN. This correlates with an alteration of the tetrameric structure of the protein, as indicated by sucrose gradient sedimentation analyses. Finally, N-glycan addition in another region of HN (residues 124 to 152), predicted by a peptide-based analysis to mediate the interaction with F, does not significantly reduce the level of fusion, arguing strongly against this site being part of the F-interactive domain in HN. Our data support the idea that the F-interactive site on HN is defined by the stalk region of the protein.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=16378965&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1128/JVI.80.2.623-633.2006
dc.subjectAmino Acid Sequence; Animals; Cell Line; HN Protein; Molecular Sequence Data; Newcastle disease virus; Polysaccharides; Protein Structure, Tertiary; Viral Fusion Proteins; Virus Replication
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleAddition of N-glycans in the stalk of the Newcastle disease virus HN protein blocks its interaction with the F protein and prevents fusion
dc.typeJournal Article
dc.source.journaltitleJournal of virology
dc.source.volume80
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/847
dc.identifier.contextkey670503
html.description.abstract<p>Most paramyxovirus fusion (F) proteins require the coexpression of the homologous attachment (HN) protein to promote membrane fusion, consistent with the existence of a virus-specific interaction between the two proteins. Analysis of the fusion activities of chimeric HN proteins indicates that the stalk region of the HN spike determines its F protein specificity, and analysis of a panel of site-directed mutants indicates that the F-interactive site resides in this region. Here, we use the addition of oligosaccharides to further explore the role of the HN stalk in the interaction with F. N-glycans were individually added at several positions in the stalk to determine their effects on the activities of HN, as well as its structure. N-glycan addition at positions 69 and 77 in the stalk specifically blocks fusion and the HN-F interaction without affecting either HN structure or its other activities. N-glycans added at other positions in the stalk modulate activities that reside in the globular head of HN. This correlates with an alteration of the tetrameric structure of the protein, as indicated by sucrose gradient sedimentation analyses. Finally, N-glycan addition in another region of HN (residues 124 to 152), predicted by a peptide-based analysis to mediate the interaction with F, does not significantly reduce the level of fusion, arguing strongly against this site being part of the F-interactive domain in HN. Our data support the idea that the F-interactive site on HN is defined by the stalk region of the protein.</p>
dc.identifier.submissionpathgsbs_sp/847
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages623-33


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