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    Regulation of mechanical interactions between fibroblasts and the substratum by stretch-activated Ca2+ entry

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    Authors
    Munevar, Steven
    Wang, Yu-Li
    Dembo, Micah
    UMass Chan Affiliations
    Department of Physiology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2003-11-25
    Keywords
    Animals; Calcium; Calcium Channels; Cell Adhesion; Cell Movement; Fibroblasts; Focal Adhesions; Gadolinium; Mice; Microscopy, Fluorescence; NIH 3T3 Cells; Pseudopodia; Vinculin
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    http://dx.doi.org/10.1242/jcs.00795
    Abstract
    Ca2+ ions have long been implicated in regulating various aspects of cell movements. We found that stretching forces applied through flexible substrata induced increases in both intracellular Ca2+ concentration and traction forces of NIH3T3 fibroblasts. Conversely, application of gadolinium, an inhibitor of stretch-activated ion channels, or removal of extracellular free Ca2+ caused inhibition of traction forces. Gadolinium treatment also inhibited cell migration without affecting the spread morphology or protrusive activities. Local application of gadolinium to the trailing region had no detectable effect on the overall traction forces, while local application to the leading edge caused a global inhibition of traction forces and cell migration, suggesting that stretch-activated channels function primarily at the leading edge. Immunofluorescence microscopy indicated that gadolinium caused a pronounced decrease in vinculin and phosphotyrosine concentrations at focal adhesions. Our observations suggest that stretch-activated Ca2+ entry in the frontal region regulates the organization of focal adhesions and the output of mechanical forces. This mechanism probably plays an important role in sustaining cell migration and in mediating active and passive responses to mechanical signals.
    Source
    J Cell Sci. 2004 Jan 1;117(Pt 1):85-92. Epub 2003 Nov 19. Link to article on publisher's site
    DOI
    10.1242/jcs.00795
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34230
    PubMed ID
    14627625
    Related Resources
    Link to article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1242/jcs.00795
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    Morningside Graduate School of Biomedical Sciences Scholarly Publications

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