HIV-1 Env glycoproteins from two series of primary isolates: replication phenotype and immunogenicity
Authors
Mustafa, FarahRichmond, Joan F. L.
Fernandez-Larsson, Roberto
Lu, Shan
Fredriksson, Robert
Fenyo, Eva Maria
O'Connell, Maryellen
Johnson, Eric
Weng, Jiayu
Santoro, Joseph C.
Robinson, Harriet L.
Document Type
Journal ArticlePublication Date
1997-03-03Keywords
AIDS Vaccines; Amino Acid Sequence; Cloning, Molecular; Gene Products, env; HIV Envelope Protein gp120; HIV-1; Humans; Male; Molecular Sequence Data; Phenotype; Plasmids; Virus Replication; env Gene Products, Human Immunodeficiency VirusLife Sciences
Medicine and Health Sciences
Metadata
Show full item recordAbstract
Seven envelope regions from two series of patient isolates have been molecularly cloned and analyzed for replication phenotypes and immunogenicity. Growth potential was analyzed for env sequences substituted into an HIV-1-NL4-3 backbone (NL4-3/env recombinants). Immunogenicity studies were conducted on secreted monomeric (gp120) and oligomeric (gp140) forms of the Envs using Env-expressing plasmid DNAs for immunizations. The env regions of the patient isolates conferred a spectrum of replication kinetics and cytotropisms on the NL4-3/env recombinants. Both patient series included non-syncytium-inducing viruses with no ability to grow on T-cell lines, and highly syncytium inducing viruses which grew well on T-cell lines. These differences in growth potential did not correlate with the ability of the DNA-expressed Envs to raise antibody in rabbits. Rather, the relative immunogenicity of the Envs was patient and form specific. The Envs from patient 5 raised higher titers of antibody than the Envs from patient 6. For each primary Env, the gp120 form of the Env raised higher titers of antibody than the gp140 form. Thus, structural features of Env that affect replication do not necessarily affect the ability to raise antibody.Source
Virology. 1997 Mar 3;229(1):269-78. Link to article on publisher's siteDOI
10.1006/viro.1997.8445Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34235PubMed ID
9123870Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1006/viro.1997.8445