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    Sorting of beta-actin mRNA and protein to neurites and growth cones in culture

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    Authors
    Bassell, Gary J.
    Zhang, Honglai
    Byrd, Anne L.
    Femino, Andrea M.
    Singer, Robert H.
    Taneja, Krishan L.
    Lifshitz, Lawrence M.
    Herman, Ira M.
    Kosik, Kenneth S.
    UMass Chan Affiliations
    Department of Physiology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    1998-01-24
    Keywords
    Actins; Amino Acid Sequence; Animals; Axonal Transport; Base Sequence; Cells, Cultured; Cerebral Cortex; In Situ Hybridization; Microscopy, Electron; Microtubules; Molecular Sequence Data; Neurites; Neurons; Polyribosomes; RNA, Messenger; Rats
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://doi.org/10.1523/JNEUROSCI.18-01-00251.1998
    Abstract
    The transport of mRNAs into developing dendrites and axons may be a basic mechanism to localize cytoskeletal proteins to growth cones and influence microfilament organization. Using isoform-specific antibodies and probes for in situ hybridization, we observed distinct localization patterns for beta- and gamma-actin within cultured cerebrocortical neurons. beta-Actin protein was highly enriched within growth cones and filopodia, in contrast to gamma-actin protein, which was distributed uniformly throughout the cell. beta-Actin protein also was shown to be peripherally localized after transfection of beta-actin cDNA bearing an epitope tag. beta-Actin mRNAs were localized more frequently to neuronal processes and growth cones, unlike gamma-actin mRNAs, which were restricted to the cell body. The rapid localization of beta-actin mRNA, but not gamma-actin mRNA, into processes and growth cones could be induced by dibutyryl cAMP treatment. Using high-resolution in situ hybridization and image-processing methods, we showed that the distribution of beta-actin mRNA within growth cones was statistically nonrandom and demonstrated an association with microtubules. beta-Actin mRNAs were detected within minor neurites, axonal processes, and growth cones in the form of spatially distinct granules that colocalized with translational components. Ultrastructural analysis revealed polyribosomes within growth cones that colocalized with cytoskeletal filaments. The transport of beta-actin mRNA into developing neurites may be a sequence-specific mechanism to synthesize cytoskeletal proteins directly within processes and growth cones and would provide an additional means to deliver cytoskeletal proteins over long distances.
    Source

    J Neurosci. 1998 Jan 1;18(1):251-65.

    DOI
    10.1523/JNEUROSCI.18-01-00251.1998
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34245
    PubMed ID
    9412505
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    Link to article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1523/JNEUROSCI.18-01-00251.1998
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