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dc.contributor.authorOrdway, Richard W.
dc.contributor.authorPetrou, Steven
dc.contributor.authorKirber, Michael T.
dc.contributor.authorWalsh, John V.
dc.contributor.authorSinger, Joshua J.
dc.date2022-08-11T08:09:02.000
dc.date.accessioned2022-08-23T16:16:10Z
dc.date.available2022-08-23T16:16:10Z
dc.date.issued1995-04-15
dc.date.submitted2008-11-24
dc.identifier.citation<p>J Physiol. 1995 Apr 15;484 ( Pt 2):331-7.</p>
dc.identifier.issn0022-3751 (Print)
dc.identifier.doi10.1113/jphysiol.1995.sp020668
dc.identifier.pmid7602529
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34268
dc.description.abstract1. using standard single channel patch clamp techniques we studied the stretch sensitivity of a 20 pS K(+)-selective channel which is activated by fatty acids and found in freshly dissociated smooth muscle cells from the stomach of the toad Bufo marinus. 2. A pulse of suction applied to the back of the patch pipette in order to stretch the membrane resulted in activation of this K+ channel. A train of suction pulses resulted in a gradually increased level of channel activity during each successive pulse, as well as an increase in baseline activity between pulses. This pattern contrasts markedly with many other stretch-activated channels whose activation is limited to the duration of the suction pulse. 3. Application of fatty acids augmented the response to stretch. In contrast, application of 10 microM defatted albumin, which removes fatty acids from membranes, rapidly and reversibly decreased the response to stretch. 4. These results are consistent with the hypothesis that fatty acids which are generated by mechanical stimuli, perhaps by mechanically activated phospholipases, are the intermediaries in activation of certain mechanically sensitive ion channels.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=7602529&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC1157897/
dc.subjectAlbumins; Animals; Bufo marinus; Fatty Acids; Membrane Potentials; Muscle, Smooth; Myristic Acid; Myristic Acids; Patch-Clamp Techniques; Physical Stimulation; Potassium Channels; Time Factors
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleStretch activation of a toad smooth muscle K+ channel may be mediated by fatty acids
dc.typeJournal Article
dc.source.journaltitleThe Journal of physiology
dc.source.volume484 ( Pt 2)
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/922
dc.identifier.contextkey671853
html.description.abstract<p>1. using standard single channel patch clamp techniques we studied the stretch sensitivity of a 20 pS K(+)-selective channel which is activated by fatty acids and found in freshly dissociated smooth muscle cells from the stomach of the toad Bufo marinus. 2. A pulse of suction applied to the back of the patch pipette in order to stretch the membrane resulted in activation of this K+ channel. A train of suction pulses resulted in a gradually increased level of channel activity during each successive pulse, as well as an increase in baseline activity between pulses. This pattern contrasts markedly with many other stretch-activated channels whose activation is limited to the duration of the suction pulse. 3. Application of fatty acids augmented the response to stretch. In contrast, application of 10 microM defatted albumin, which removes fatty acids from membranes, rapidly and reversibly decreased the response to stretch. 4. These results are consistent with the hypothesis that fatty acids which are generated by mechanical stimuli, perhaps by mechanically activated phospholipases, are the intermediaries in activation of certain mechanically sensitive ion channels.</p>
dc.identifier.submissionpathgsbs_sp/922
dc.contributor.departmentDepartment of Physiology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages331-7


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