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    Characterization of an alternate form of Newcastle disease virus fusion protein

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    Authors
    Pantua, Homer Dadios
    McGinnes, Lori
    Leszyk, John D.
    Morrison, Trudy G.
    UMass Chan Affiliations
    Department of Biochemistry and Molecular Pharmacology
    Department of Molecular Genetics and Microbiology
    Program in Immunology and Virology
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2005-09-06
    Keywords
    Amino Acid Sequence; Animals; Antibodies, Viral; COS Cells; Cercopithecus aethiops; Chickens; Molecular Sequence Data; Newcastle disease virus; Protein Isoforms; Rabbits; Recombinant Proteins; Transfection; Viral Fusion Proteins
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    http://dx.doi.org/10.1128/JVI.79.18.11660-11670.2005
    Abstract
    The sequence and structure of the Newcastle disease virus (NDV) fusion (F) protein are consistent with its classification as a type 1 glycoprotein. We have previously reported, however, that F protein can be detected in at least two topological forms with respect to membranes in both a cell-free protein synthesizing system containing membranes and infected COS-7 cells (J. Virol. 77:1951-1963, 2003). One form is the classical type 1 glycoprotein, while the other is a polytopic form in which approximately 200 amino acids of the amino-terminal end as well as the cytoplasmic domain (CT) are translocated across membranes. Furthermore, we detected CT sequences on surfaces of F protein-expressing cells, and antibodies specific for these sequences inhibited red blood cell fusion to hemagglutinin-neuraminidase and F protein-expressing cells, suggesting a role for surface-expressed CT sequences in cell-cell fusion. Extending these findings, we have found that the alternate form of the F protein can also be detected in infected and transfected avian cells, the natural host cells of NDV. Furthermore, the alternate form of the F protein was also found in virions released from both infected COS-7 cells and avian cells by Western analysis. Mass spectrometry confirmed its presence in virions released from avian cells. Two different polyclonal antibodies raised against sequences of the CT domain of the F protein slowed plaque formation in both avian and COS-7 cells. Antibody specific for the CT domain also inhibited single-cycle infections, as detected by immunofluorescence of viral proteins in infected cells. The potential roles of this alternate form of the NDV F protein in infection are discussed.
    Source
    J Virol. 2005 Sep;79(18):11660-70. Link to article on publisher's site
    DOI
    10.1128/JVI.79.18.11660-11670.2005
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34277
    PubMed ID
    16140743
    Related Resources
    Link to article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1128/JVI.79.18.11660-11670.2005
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