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dc.contributor.authorWentworth, Bruce Martin
dc.contributor.authorSchaefer, Ida M.
dc.contributor.authorVilla-Komaroff, Lydia
dc.contributor.authorChirgwin, John M.
dc.date2022-08-11T08:09:02.000
dc.date.accessioned2022-08-23T16:16:13Z
dc.date.available2022-08-23T16:16:13Z
dc.date.issued1986-01-01
dc.date.submitted2008-11-25
dc.identifier.citationJ Mol Evol. 1986;23(4):305-12.
dc.identifier.issn0022-2844 (Print)
dc.identifier.pmid3104603
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34279
dc.description.abstractWe have cloned and sequenced the two mouse preproinsulin genes. The deduced amino acid sequences of the mature mouse insulins are identical to the published protein sequences. However, the nucleotide sequence indicates that the mouse I C-peptide has a deletion of two amino acids compared with the mouse II C-peptide. We used an S1 nuclease assay to confirm the presence of the deletion and to measure the ratio of transcripts from gene I to transcripts from gene II. The mouse preproinsulin I gene, like the rat gene I, is missing the second intervening sequence that normally interrupts the C-peptide region in other insulin genes. Comparison of the 5' flanking sequences of the mouse and rat genes II indicates that they are homologous for at least 1000 base pairs. The preproinsulin I genes also share homology in their 5' flanking DNAs; however, their homology to the preproinsulin II genes extends for only about 500 base pairs.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=3104603&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1007/BF02100639
dc.subjectAmino Acid Sequence; Animals; Base Sequence; Cloning, Molecular; *Genes; Mice; Mice, Inbred BALB C; Mice, Inbred C57BL; Proinsulin; Protein Precursors; Templates, Genetic
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleCharacterization of the two nonallelic genes encoding mouse preproinsulin
dc.typeJournal Article
dc.source.journaltitleJournal of molecular evolution
dc.source.volume23
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/932
dc.identifier.contextkey672205
html.description.abstract<p>We have cloned and sequenced the two mouse preproinsulin genes. The deduced amino acid sequences of the mature mouse insulins are identical to the published protein sequences. However, the nucleotide sequence indicates that the mouse I C-peptide has a deletion of two amino acids compared with the mouse II C-peptide. We used an S1 nuclease assay to confirm the presence of the deletion and to measure the ratio of transcripts from gene I to transcripts from gene II. The mouse preproinsulin I gene, like the rat gene I, is missing the second intervening sequence that normally interrupts the C-peptide region in other insulin genes. Comparison of the 5' flanking sequences of the mouse and rat genes II indicates that they are homologous for at least 1000 base pairs. The preproinsulin I genes also share homology in their 5' flanking DNAs; however, their homology to the preproinsulin II genes extends for only about 500 base pairs.</p>
dc.identifier.submissionpathgsbs_sp/932
dc.contributor.departmentDepartment of Molecular Genetics and Microbiology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages305-12


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