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    Mitochondrial biogenesis and remodeling during adipogenesis and in response to the insulin sensitizer rosiglitazone

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    Authors
    Wilson-Fritch, Leanne
    Burkart, Alison
    Bell, Gregory
    Mendelson, Karen
    Leszyk, John D.
    Nicoloro, Sarah M.
    Czech, Michael P.
    Corvera, Silvia
    UMass Chan Affiliations
    Department of Biochemistry and Molecular Pharmacology
    Program in Molecular Medicine
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2003-01-17
    Keywords
    3T3 Cells; Adipocytes; Animals; Cell Differentiation; Insulin; Mice; Microscopy, Electron; Mitochondria; Mitochondrial Proteins; Oxygen Consumption; RNA, Messenger; Thiazoles; *Thiazolidinediones
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    https://www.ncbi.nlm.nih.gov/pmc/articles/PMC140688/
    Abstract
    White adipose tissue is an important endocrine organ involved in the control of whole-body metabolism, insulin sensitivity, and food intake. To better understand these functions, 3T3-L1 cell differentiation was studied by using combined proteomic and genomic strategies. The proteomics approach developed here exploits velocity gradient centrifugation as an alternative to isoelectric focusing for protein separation in the first dimension. A 20- to 30-fold increase in the concentration of numerous mitochondrial proteins was observed during adipogenesis, as determined by mass spectrometry and database correlation analysis. Light and electron microscopy confirmed a large increase in the number of mitochondrion profiles with differentiation. Furthermore, mRNA profiles obtained by using Affymetrix GeneChips revealed statistically significant increases in the expression of many nucleus-encoded mitochondrial genes during adipogenesis. Qualitative changes in mitochondrial composition also occur during adipose differentiation, as exemplified by increases in expression of proteins involved in fatty acid metabolism and of mitochondrial chaperones. Furthermore, the insulin sensitizer rosiglitazone caused striking changes in mitochondrial shape and expression of selective mitochondrial proteins. Thus, although mitochondrial biogenesis has classically been associated with brown adipocyte differentiation and thermogenesis, our results reveal that mitochondrial biogenesis and remodeling are inherent to adipose differentiation per se and are influenced by the actions of insulin sensitizers.
    Source

    Mol Cell Biol. 2003 Feb;23(3):1085-94.

    DOI
    10.1128/MCB.23.3.1085-1094.2003
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34284
    PubMed ID
    12529412
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    Link to Article in PubMed

    ae974a485f413a2113503eed53cd6c53
    10.1128/MCB.23.3.1085-1094.2003
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