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    The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells

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    Authors
    Parish, Joanna L.
    Rosa, Jack
    Wang, Xiaoyu
    Lahti, Jill M.
    Doxsey, Stephen J.
    Androphy, Elliot J.
    UMass Chan Affiliations
    Program in Molecular Medicine
    Department of Medicine
    Graduate School of Biomedical Sciences
    Document Type
    Journal Article
    Publication Date
    2006-11-16
    Keywords
    Animals; Chromosome Segregation; DEAD-box RNA Helicases; DNA Helicases; Hela Cells; Humans; Mitotic Spindle Apparatus; Mutation; Prometaphase; Protein Binding; Sister Chromatid Exchange; Tissue Distribution
    Life Sciences
    Medicine and Health Sciences
    
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    Link to Full Text
    http://dx.doi.org/10.1242/jcs.03262
    Abstract
    It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.
    Source
    J Cell Sci. 2006 Dec 1;119(Pt 23):4857-65. Epub 2006 Nov 14. Link to article on publisher's site
    DOI
    10.1242/jcs.03262
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/34307
    PubMed ID
    17105772
    Related Resources
    Link to article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1242/jcs.03262
    Scopus Count
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    Morningside Graduate School of Biomedical Sciences Scholarly Publications

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