The DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells
UMass Chan Affiliations
Program in Molecular MedicineDepartment of Medicine
Graduate School of Biomedical Sciences
Document Type
Journal ArticlePublication Date
2006-11-16Keywords
Animals; Chromosome Segregation; DEAD-box RNA Helicases; DNA Helicases; Hela Cells; Humans; Mitotic Spindle Apparatus; Mutation; Prometaphase; Protein Binding; Sister Chromatid Exchange; Tissue DistributionLife Sciences
Medicine and Health Sciences
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Show full item recordAbstract
It has recently been suggested that the Saccharomyces cerevisiae protein Chl1p plays a role in cohesion establishment. Here, we show that the human ATP-dependent DNA helicase ChlR1 is required for sister chromatid cohesion in mammalian cells. Localization studies show that ChlR1 diffusely coats mitotic chromatin in prophase and then translocates from the chromatids to concentrate at the spindle poles during the transition to metaphase. Depletion of ChlR1 protein by RNA interference results in mitotic failure with replicated chromosomes failing to segregate after a pro-metaphase arrest. We show that depletion also results in abnormal sister chromatid cohesion, determined by increased separation of chromatid pairs at the centromere. Furthermore, biochemical studies show that ChlR1 is in complex with cohesin factors Scc1, Smc1 and Smc3. We conclude that human ChlR1 is required for sister chromatid cohesion and, hence, normal mitotic progression. These functions are important to maintain genetic fidelity.Source
J Cell Sci. 2006 Dec 1;119(Pt 23):4857-65. Epub 2006 Nov 14. Link to article on publisher's siteDOI
10.1242/jcs.03262Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34307PubMed ID
17105772Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1242/jcs.03262