A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation
dc.contributor.author | Parker, Breck Olland | |
dc.contributor.author | Marinus, Martin G. | |
dc.date | 2022-08-11T08:09:02.000 | |
dc.date.accessioned | 2022-08-23T16:16:20Z | |
dc.date.available | 2022-08-23T16:16:20Z | |
dc.date.issued | 1988-12-20 | |
dc.date.submitted | 2008-11-25 | |
dc.identifier.citation | Gene. 1988 Dec 20;73(2):531-5. | |
dc.identifier.issn | 0378-1119 (Print) | |
dc.identifier.pmid | 2854098 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/34308 | |
dc.description.abstract | We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2854098&dopt=Abstract">Link to article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1016/0378-1119(88)90517-3 | |
dc.subject | *Chromosome Deletion; Chromosome Mapping; Chromosomes, Bacterial; Crosses, Genetic; *DNA Transposable Elements; Escherichia coli; *Genes; *Genes, Bacterial; Genotype; *Mutation; Site-Specific DNA-Methyltransferase (Adenine-Specific); Transduction, Genetic | |
dc.subject | Life Sciences | |
dc.subject | Medicine and Health Sciences | |
dc.title | A simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation | |
dc.type | Journal Article | |
dc.source.journaltitle | Gene | |
dc.source.volume | 73 | |
dc.source.issue | 2 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/gsbs_sp/959 | |
dc.identifier.contextkey | 672249 | |
html.description.abstract | <p>We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli.</p> | |
dc.identifier.submissionpath | gsbs_sp/959 | |
dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
dc.contributor.department | Graduate School of Biomedical Sciences | |
dc.source.pages | 531-5 |