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dc.contributor.authorParker, Breck Olland
dc.contributor.authorMarinus, Martin G.
dc.date2022-08-11T08:09:02.000
dc.date.accessioned2022-08-23T16:16:20Z
dc.date.available2022-08-23T16:16:20Z
dc.date.issued1988-12-20
dc.date.submitted2008-11-25
dc.identifier.citationGene. 1988 Dec 20;73(2):531-5.
dc.identifier.issn0378-1119 (Print)
dc.identifier.pmid2854098
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34308
dc.description.abstractWe describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2854098&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/0378-1119(88)90517-3
dc.subject*Chromosome Deletion; Chromosome Mapping; Chromosomes, Bacterial; Crosses, Genetic; *DNA Transposable Elements; Escherichia coli; *Genes; *Genes, Bacterial; Genotype; *Mutation; Site-Specific DNA-Methyltransferase (Adenine-Specific); Transduction, Genetic
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleA simple and rapid method to obtain substitution mutations in Escherichia coli: isolation of a dam deletion/insertion mutation
dc.typeJournal Article
dc.source.journaltitleGene
dc.source.volume73
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/959
dc.identifier.contextkey672249
html.description.abstract<p>We describe the isolation of a strain of Escherichia coli bearing a deletion/insertion (i.e., a substitution mutation) in the dam gene (dam-16). The mutagenesis protocol used should be applicable to any cloned non-essential gene of E. coli. The substitution mutation confers resistance to kanamycin and can easily be transferred to other strains by standard genetic techniques. The amount of Dam methyltransferase (MTase) in dam-16 strains as determined either in vitro or in vivo is below the level of detection. We conclude that the Dam MTase is not required for viability of E. coli.</p>
dc.identifier.submissionpathgsbs_sp/959
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages531-5


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