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dc.contributor.authorPertmer, Tamera Marie
dc.contributor.authorOran, Alp E.
dc.contributor.authorMadorin, Catherine A.
dc.contributor.authorRobinson, Harriet L.
dc.date2022-08-11T08:09:02.000
dc.date.accessioned2022-08-23T16:16:26Z
dc.date.available2022-08-23T16:16:26Z
dc.date.issued2001-02-13
dc.date.submitted2008-11-25
dc.identifier.citation<p>Vaccine. 2001 Feb 8;19(13-14):1764-71.</p>
dc.identifier.issn0264-410X (Print)
dc.identifier.doi10.1016/S0264-410X(00)00388-1
dc.identifier.pmid11166902
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34332
dc.description.abstractIn these studies, we address the ability of DNA encoding Th1 cytokines to bias the isotype of antibody raised by neonatal or adult immunization with an influenza hemagglutinin expressing DNA (HA-DNA). Neonatal mice coimmunized with HA-DNA and either IL-12 or IFN-gamma-expressing DNA developed IgG2a-biased immune responses, regardless of inoculation method. In contrast, the Th1 genetic adjuvants had no effect on IgG subtype patterns in adults. In neonatal mice, the Th1 genetic adjuvants also shifted the pattern of lymphokine production by recall splenocytes from a mixed response of IFN-gamma and IL-5 to exclusively IFN-gamma. In adults, despite the failure to change the isotype pattern of the antibody response, a shift towards IFN-gamma production also occurred for recall splenocytes following coimmunzation with IL-12. Thus, coinoculation of Th1 genetic adjuvants had greater effects on the nature of the immune response in the neonate than in adults.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11166902&dopt=Abstract">Link to article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1016/S0264-410X(00)00388-1
dc.subjectAdjuvants, Immunologic; Aging; Animals; Animals, Newborn; Enzyme-Linked Immunosorbent Assay; Hemagglutinin Glycoproteins, Influenza Virus; Immunoglobulin G; Immunoglobulin Isotypes; Interferon Type II; Interleukin-12; Interleukin-5; Mice; Mice, Inbred BALB C; Spleen; Th1 Cells; Vaccination; Vaccines, DNA
dc.subjectLife Sciences
dc.subjectMedicine and Health Sciences
dc.titleTh1 genetic adjuvants modulate immune responses in neonates
dc.typeJournal Article
dc.source.journaltitleVaccine
dc.source.volume19
dc.source.issue13-14
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/gsbs_sp/980
dc.identifier.contextkey672508
html.description.abstract<p>In these studies, we address the ability of DNA encoding Th1 cytokines to bias the isotype of antibody raised by neonatal or adult immunization with an influenza hemagglutinin expressing DNA (HA-DNA). Neonatal mice coimmunized with HA-DNA and either IL-12 or IFN-gamma-expressing DNA developed IgG2a-biased immune responses, regardless of inoculation method. In contrast, the Th1 genetic adjuvants had no effect on IgG subtype patterns in adults. In neonatal mice, the Th1 genetic adjuvants also shifted the pattern of lymphokine production by recall splenocytes from a mixed response of IFN-gamma and IL-5 to exclusively IFN-gamma. In adults, despite the failure to change the isotype pattern of the antibody response, a shift towards IFN-gamma production also occurred for recall splenocytes following coimmunzation with IL-12. Thus, coinoculation of Th1 genetic adjuvants had greater effects on the nature of the immune response in the neonate than in adults.</p>
dc.identifier.submissionpathgsbs_sp/980
dc.contributor.departmentProgram in Immunology and Virology
dc.contributor.departmentGraduate School of Biomedical Sciences
dc.source.pages1764-71


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