Authors
Harada, AkihitoOkada, Seiji
Konno, Daijiro
Odawara, Jun
Yoshimi, Tomohiko
Yoshimura, Saori
Kumamaru, Hiromi
Saiwai, Hirokazu
Tsubota, Toshiaki
Kurumizaka, Hitoshi
Akashi, Koichi
Tachibana, Taro
Imbalzano, Anthony N.
Ohkawa, Yasuyuki
UMass Chan Affiliations
Department of Cell and Developmental BiologyDocument Type
Journal ArticlePublication Date
2012-05-08Keywords
AnimalsCell Line
DNA-Binding Proteins
Gene Expression Regulation, Developmental
Gene Knockdown Techniques
Genetic Loci
Histones
Mice
*Muscle Development
MyoD Protein
Transcriptional Activation
Cell and Developmental Biology
Cell Biology
Metadata
Show full item recordAbstract
Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.Source
EMBO J. 2012 May 8;31(13):2994-3007. doi: 10.1038/emboj.2012.136. Link to article on publisher's siteDOI
10.1038/emboj.2012.136Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34856PubMed ID
22569126Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/emboj.2012.136