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dc.contributor.authorHarada, Akihito
dc.contributor.authorOkada, Seiji
dc.contributor.authorKonno, Daijiro
dc.contributor.authorOdawara, Jun
dc.contributor.authorYoshimi, Tomohiko
dc.contributor.authorYoshimura, Saori
dc.contributor.authorKumamaru, Hiromi
dc.contributor.authorSaiwai, Hirokazu
dc.contributor.authorTsubota, Toshiaki
dc.contributor.authorKurumizaka, Hitoshi
dc.contributor.authorAkashi, Koichi
dc.contributor.authorTachibana, Taro
dc.contributor.authorImbalzano, Anthony N.
dc.contributor.authorOhkawa, Yasuyuki
dc.date2022-08-11T08:09:08.000
dc.date.accessioned2022-08-23T16:18:36Z
dc.date.available2022-08-23T16:18:36Z
dc.date.issued2012-05-08
dc.date.submitted2012-10-02
dc.identifier.citationEMBO J. 2012 May 8;31(13):2994-3007. doi: 10.1038/emboj.2012.136. <a href="http://dx.doi.org/10.1038/emboj.2012.136">Link to article on publisher's site</a>
dc.identifier.issn0261-4189 (Linking)
dc.identifier.doi10.1038/emboj.2012.136
dc.identifier.pmid22569126
dc.identifier.urihttp://hdl.handle.net/20.500.14038/34856
dc.description.abstractCell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22569126&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1038/emboj.2012.136
dc.subjectAnimals
dc.subjectCell Line
dc.subjectDNA-Binding Proteins
dc.subjectGene Expression Regulation, Developmental
dc.subjectGene Knockdown Techniques
dc.subjectGenetic Loci
dc.subjectHistones
dc.subjectMice
dc.subject*Muscle Development
dc.subjectMyoD Protein
dc.subjectTranscriptional Activation
dc.subjectCell and Developmental Biology
dc.subjectCell Biology
dc.titleChd2 interacts with H3.3 to determine myogenic cell fate
dc.typeJournal Article
dc.source.journaltitleThe EMBO journal
dc.source.volume31
dc.source.issue13
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/imbalzano/16
dc.identifier.contextkey3363199
html.description.abstract<p>Cell differentiation is mediated by lineage-determining transcription factors. We show that chromodomain helicase DNA-binding domain 2 (Chd2), a SNF2 chromatin remodelling enzyme family member, interacts with MyoD and myogenic gene regulatory sequences to specifically mark these loci via deposition of the histone variant H3.3 prior to cell differentiation. Directed and genome-wide analysis of endogenous H3.3 incorporation demonstrates that knockdown of Chd2 prevents H3.3 deposition at differentiation-dependent, but not housekeeping, genes and inhibits myogenic gene activation. The data indicate that MyoD determines cell fate and facilitates differentiation-dependent gene expression through Chd2-dependent deposition of H3.3 at myogenic loci prior to differentiation.</p>
dc.identifier.submissionpathimbalzano/16
dc.contributor.departmentDepartment of Cell and Developmental Biology
dc.source.pages2994-3007


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