Ectopic runx2 expression in mammary epithelial cells disrupts formation of normal acini structure: implications for breast cancer progression
Imbalzano, Karen M.
Underwood, Jean. M.
Gokul, Karthiga Devi
Van Wijnen, Andre J.
Stein, Janet L.
Imbalzano, Anthony N.
Nickerson, Jeffrey A.
Lian, Jane B.
Stein, Gary S.
UMass Chan AffiliationsDepartment of Cell Biology
Cell Culture Techniques
*Cell Transformation, Neoplastic
Core Binding Factor Alpha 1 Subunit
*Gene Expression Regulation, Neoplastic
*Mammary Glands, Human
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AbstractThe transcription factor Runx2 is highly expressed in breast cancer cells compared with mammary epithelial cells and contributes to metastasis. Here we directly show that Runx2 expression promotes a tumor cell phenotype of mammary acini in three-dimensional culture. Human mammary epithelial cells (MCF-10A) form polarized, growth-arrested, acini-like structures with glandular architecture. The ectopic expression of Runx2 disrupts acini formation, and electron microscopic ultrastructural analysis revealed the absence of lumens. Characterization of the disrupted acini structures showed increased cell proliferation (Ki-67 positive cells), decreased apoptosis (Bcl-2 induction), and loss of basement membrane formation (absence of beta(4) integrin expression). In complementary experiments, inhibition of Runx2 function in metastatic MDA-MB-231 breast cancer cells by stable expression of either short hairpin RNA-Runx2 or a mutant Runx2 deficient in subnuclear targeting resulted in reversion of acini to more normal structures and reduced tumor growth in vivo. These novel findings provide direct mechanistic evidence for the biological activity of Runx2, dependent on its subnuclear localization, in promoting early events of breast cancer progression and suggest a molecular therapeutic target.
SourceCancer Res. 2009 Sep 1;69(17):6807-14. Epub 2009 Aug 18. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/34862
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