The expression of myogenic microRNAs indirectly requires protein arginine methyltransferase (Prmt)5 but directly requires Prmt4
Authors
Mallappa, ChandrashekaraHu, Yu-Jie
Shamulailatpam, Priscilla
Tae, Sookil
Sif, Said
Imbalzano, Anthony N.
UMass Chan Affiliations
Department of Cell BiologyDocument Type
Journal ArticlePublication Date
2011-03-17
Metadata
Show full item recordAbstract
Myogenic microRNAs are important regulators of muscle development and differentiation. To better understand the roles of chromatin-modifying and remodeling enzymes in the activation of myogenic microRNA expression, we have functionally analyzed two different protein arginine methyltransferases, Prmt5 and Prmt4, both of which have previously been implicated in the regulation of myogenic mRNA expression. Both Prmts are required for myogenic microRNA induction during differentiation. Prmt5 is indirectly required due to the necessity of Prmt5 for expression of the transcriptional regulator, myogenin, as ectopic expression of myogenin eliminates Prmt5 dependency. By contrast, Prmt4 binds to the upstream regulatory regions of myogenic microRNAs and is required for dimethylation of the Prmt4 substrate, H3R17, at microRNA regulatory sequences. Deletion of Prmt4 does not alter MyoD binding at myogenic microRNA regulatory sequences but prevents the binding of both myogenin and the Brg1 ATPase that catalyzes SWI/SNF-dependent chromatin remodeling, resulting in an inhibition of microRNA expression.Source
Nucleic Acids Res. 2011 Mar 1;39(4):1243-55. Epub 2010 Oct 14. doi: 10.1093/nar/gkq896. Link to article on publisher's siteDOI
10.1093/nar/gkq896Permanent Link to this Item
http://hdl.handle.net/20.500.14038/34864PubMed ID
20947566Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1093/nar/gkq896