Rift Valley fever virus infection induces activation of the NLRP3 inflammasome
AuthorsErmler, Megan E.
Schattgen, Stefan A.
Vanaja, Sivapriya K.
Fitzgerald, Katherine A.
Hise, Amy G.
UMass Chan AffiliationsDepartment of Medicine, Division of Infectious Diseases and Immunology
Document TypeJournal Article
KeywordsAdaptor Proteins, Signal Transducing
Mice, Inbred C57BL
Rift Valley Fever
Rift Valley fever virus
Amino Acids, Peptides, and Proteins
Immunology and Infectious Disease
Immunology of Infectious Disease
MetadataShow full item record
AbstractInflammasome activation is gaining recognition as an important mechanism for protection during viral infection. Here, we investigate whether Rift Valley fever virus, a negative-strand RNA virus, can induce inflammasome responses and IL-1beta processing in immune cells. We have determined that RVFV induces NLRP3 inflammasome activation in murine dendritic cells, and that this process is dependent upon ASC and caspase-1. Furthermore, absence of the cellular RNA helicase adaptor protein MAVS/IPS-1 significantly reduces extracellular IL-1beta during infection. Finally, direct imaging using confocal microscopy shows that the MAVS protein co-localizes with NLRP3 in the cytoplasm of RVFV infected cells.
SourceVirology. 2014 Jan 20;449:174-80. doi: 10.1016/j.virol.2013.11.015. Epub 2013 Dec 3. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/34963
Related ResourcesLink to Article in PubMed