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Peptidoglycan induces loss of a nuclear peptidoglycan recognition protein during host tissue development in a beneficial animal-bacterial symbiosis
Authors
Troll, Joshua V.Adin, Dawn M.
Wier, Andrew M.
Paquette, Nicholas Paul
Silverman, Neal
Goldman, William E.
Stadermann, Frank J.
Stabb, Eric V.
McFall-Ngai, Margaret J.
UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyDocument Type
Journal ArticlePublication Date
2009-05-07Keywords
Amino Acid SequenceAnimals
Apoptosis
Carrier Proteins
Cell Nucleus
Decapodiformes
Epithelial Cells
Gene Deletion
Microscopy, Fluorescence
Molecular Sequence Data
Peptidoglycan
*Symbiosis
Vibrio fischeri
Immunology and Infectious Disease
Metadata
Show full item recordAbstract
Peptidoglycan recognition proteins (PGRPs) are mediators of innate immunity and recently have been implicated in developmental regulation. To explore the interplay between these two roles, we characterized a PGRP in the host squid Euprymna scolopes (EsPGRP1) during colonization by the mutualistic bacterium Vibrio fischeri. Previous research on the squid-vibrio symbiosis had shown that, upon colonization of deep epithelium-lined crypts of the host light organ, symbiont-derived peptidoglycan monomers induce apoptosis-mediated regression of remote epithelial fields involved in the inoculation process. In this study, immunofluorescence microscopy revealed that EsPGRP1 localizes to the nuclei of epithelial cells, and symbiont colonization induces the loss of EsPGRP1 from apoptotic nuclei. The loss of nuclear EsPGRP1 occurred prior to DNA cleavage and breakdown of the nuclear membrane, but followed chromatin condensation, suggesting that it occurs during late-stage apoptosis. Experiments with purified peptidoglycan monomers and with V. fischeri mutants defective in peptidoglycan-monomer release provided evidence that these molecules trigger nuclear loss of EsPGRP1 and apoptosis. The demonstration of a nuclear PGRP is unprecedented, and the dynamics of EsPGRP1 during apoptosis provide a striking example of a connection between microbial recognition and developmental responses in the establishment of symbiosis.Source
Cell Microbiol. 2009 Jul;11(7):1114-27. Epub 2009 Mar 12. Link to article on publisher's siteDOI
10.1111/j.1462-5822.2009.01315.xPermanent Link to this Item
http://hdl.handle.net/20.500.14038/34969PubMed ID
19416268Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1111/j.1462-5822.2009.01315.x