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    A human CD4+ T cell epitope in the influenza hemagglutinin is cross-reactive to influenza A virus subtypes and to influenza B virus

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    J._Virol._2012_Babon_9233_43.pdf
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    Authors
    Babon, Jenny Aurielle B.
    Cruz, John
    Ennis, Francis A.
    Yin, Liusong
    Terajima, Masanori
    UMass Chan Affiliations
    Department of Pathology
    Graduate School of Biomedical Sciences, Program in Immunology and Virology
    Division of Infectious Diseases and Immunology, Department of Medicine
    Document Type
    Journal Article
    Publication Date
    2012-09-01
    Keywords
    Immunity
    Immunology and Infectious Disease
    Immunology of Infectious Disease
    Infectious Disease
    
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    Abstract
    The hemagglutinin protein (HA) of the influenza virus family is a major antigen for protective immunity. Thus, it is a relevant target for developing vaccines. Here, we describe a human CD4(+) T cell epitope in the influenza virus HA that lies in the fusion peptide of the HA. This epitope is well conserved in all 16 subtypes of the HA protein of influenza A virus and the HA protein of influenza B virus. By stimulating peripheral blood mononuclear cells (PBMCs) from a healthy adult donor with peptides covering the entire HA protein based on the sequence of A/Japan/305/1957 (H2N2), we generated a T cell line specific to this epitope. This CD4(+) T cell line recognizes target cells infected with influenza A virus seasonal H1N1 and H3N2 strains, a reassortant H2N1 strain, the 2009 pandemic H1N1 strain, and influenza B virus in cytotoxicity assays and intracellular-cytokine-staining assays. It also lysed target cells infected with avian H5N1 virus. We screened healthy adult PBMCs for T cell responses specific to this epitope and found individuals who had ex vivo gamma interferon (IFN-gamma) responses to the peptide epitope in enzyme-linked immunospot (ELISPOT) assays. Almost all donors who responded to the epitope had the HLA-DRB1*09 allele, a relatively common HLA allele. Although natural infection or standard vaccination may not induce strong T and B cell responses to this highly conserved epitope in the fusion peptide, it may be possible to develop a vaccination strategy to induce these CD4(+) T cells, which are cross-reactive to both influenza A and B viruses.
    Source
    J Virol. 2012 Sep;86(17):9233-43. doi: 10.1128/JVI.06325-11. Epub 2012 Jun 20. Link to article on publisher's site
    DOI
    10.1128/JVI.06325-11
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/35018
    PubMed ID
    22718815
    Related Resources
    Link to Article in PubMed
    Rights
    Publisher PDF posted as allowed by the publisher's author rights policy at http://journals.asm.org/site/misc/ASM_Author_Statement.xhtml.
    ae974a485f413a2113503eed53cd6c53
    10.1128/JVI.06325-11
    Scopus Count
    Collections
    Morningside Graduate School of Biomedical Sciences Scholarly Publications
    UMass Chan Faculty and Researcher Publications

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