Sequential immunization with heterologous chimeric flaviviruses induces broad-spectrum cross-reactive CD8+ T cell responses
Authors
Singh, RekhaRothman, Alan L.
Potts, James A.
Guirakhoo, Farshad
Ennis, Francis A.
Green, Sharone
UMass Chan Affiliations
Division of Infectious Diseases and Immunology, Department of MedicineCenter for Infectious Disease and Vaccine Research
Document Type
Journal ArticlePublication Date
2010-07-15Keywords
ImmunityImmunology and Infectious Disease
Immunology of Infectious Disease
Infectious Disease
Metadata
Show full item recordAbstract
Flavivirus vaccines based on ChimeriVax technology contain the nonstructural genes of the yellow fever vaccine and the premembrane and envelope genes of heterologous flaviviruses, such as Japanese encephalitis and West Nile viruses. These chimeric vaccines induce both humoral and cell-mediated immunity. Mice were vaccinated with yellow fever, chimeric Japanese encephalitis virus (YF/JE), or chimeric West Nile virus (YF/WN) vaccines, followed by a secondary homologous or heterologous vaccination; the hierarchy and function of CD8(+) T cell responses to a variable envelope epitope were then analyzed and compared with those directed against a conserved immunodominant yellow fever virus NS3 epitope. Sequential vaccination with heterologous chimeric flaviviruses generated a broadly cross-reactive CD8(+) T cell response dependent on both the sequence of infecting viruses and epitope variant. The enhanced responses to variant epitopes after heterologous vaccination were not related to preexisting antibody or to higher virus titers. These results demonstrate that the sequence of vaccination affects the expansion of cross-reactive CD8(+) T cells after heterologous chimeric flavivirus challenge.Source
J Infect Dis. 2010 Jul 15;202(2):223-33. doi: 10.1086/653486. Link to article on publisher's siteDOI
10.1086/653486Permanent Link to this Item
http://hdl.handle.net/20.500.14038/35025PubMed ID
20536361Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1086/653486