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dc.contributor.authorMangada, Marlou M.
dc.contributor.authorEnnis, Francis A.
dc.contributor.authorRothman, Alan L.
dc.date2022-08-11T08:09:09.000
dc.date.accessioned2022-08-23T16:19:28Z
dc.date.available2022-08-23T16:19:28Z
dc.date.issued2004-01-01
dc.date.submitted2017-08-22
dc.identifier.citationJ Immunol Methods. 2004 Jan;284(1-2):89-97. doi:10.1016/j.jim.2003.10.003
dc.identifier.issn0022-1759 (Linking)
dc.identifier.doi10.1016/j.jim.2003.10.003
dc.identifier.pmid14736419
dc.identifier.urihttp://hdl.handle.net/20.500.14038/35055
dc.description.abstractWe developed an intracellular cytokine staining assay to quantify dengue specific memory T cells elicited by a primary dengue virus (DEN) infection. Peripheral blood mononuclear cells (PBMC) of volunteers who received experimental live attenuated monovalent DEN vaccines were stimulated with glutaraldehyde-inactivated dengue-infected Vero cell culture lysates from all four DEN serotypes. CD4+ T cell frequencies to previously identified MHC class II peptides were equivalent to 40-70% of the responses using virus infected cell lysates in two donors. IFN-gamma responses to DEN were detected from 0.04% to 0.45% CD4+ T cells. The highest IFN-gamma response was elicited by antigens from the homologous serotype. We detected serotype-specific and -cross reactive CD4+ T cell cytokine responses from all donors. This assay is suitable for measuring DEN specific CD4+ T cells in human PBMC from large population studies where donor haplotype is unknown or highly variant.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=14736419&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttps://doi.org/10.1016/j.jim.2003.10.003
dc.subjectImmunity
dc.subjectImmunology and Infectious Disease
dc.subjectImmunology of Infectious Disease
dc.subjectInfectious Disease
dc.titleQuantitation of dengue virus specific CD4+ T cells by intracellular cytokine staining
dc.typeJournal Article
dc.source.journaltitleJournal of immunological methods
dc.source.volume284
dc.source.issue1-2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/infdis_pp/269
dc.identifier.contextkey10636645
html.description.abstract<p>We developed an intracellular cytokine staining assay to quantify dengue specific memory T cells elicited by a primary dengue virus (DEN) infection. Peripheral blood mononuclear cells (PBMC) of volunteers who received experimental live attenuated monovalent DEN vaccines were stimulated with glutaraldehyde-inactivated dengue-infected Vero cell culture lysates from all four DEN serotypes. CD4+ T cell frequencies to previously identified MHC class II peptides were equivalent to 40-70% of the responses using virus infected cell lysates in two donors. IFN-gamma responses to DEN were detected from 0.04% to 0.45% CD4+ T cells. The highest IFN-gamma response was elicited by antigens from the homologous serotype. We detected serotype-specific and -cross reactive CD4+ T cell cytokine responses from all donors. This assay is suitable for measuring DEN specific CD4+ T cells in human PBMC from large population studies where donor haplotype is unknown or highly variant.</p>
dc.identifier.submissionpathinfdis_pp/269
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.contributor.departmentCenter for Infection Disease and Vaccine Research
dc.source.pages89-97


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