Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones
dc.contributor.author | Green, Sharone | |
dc.contributor.author | Kurane, Ichiro | |
dc.contributor.author | Pincus, Steven | |
dc.contributor.author | Paoletti, Enzo | |
dc.contributor.author | Ennis, Francis A. | |
dc.date | 2022-08-11T08:09:09.000 | |
dc.date.accessioned | 2022-08-23T16:19:37Z | |
dc.date.available | 2022-08-23T16:19:37Z | |
dc.date.issued | 1997-08-04 | |
dc.date.submitted | 2017-10-02 | |
dc.identifier.citation | Virology. 1997 Aug 4;234(2):383-6. <a href="https://doi.org/10.1006/viro.1997.8648">Link to article on publisher's site</a> | |
dc.identifier.issn | 0042-6822 (Linking) | |
dc.identifier.doi | 10.1006/viro.1997.8648 | |
dc.identifier.pmid | 9268170 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/35093 | |
dc.description.abstract | We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-1-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9268170&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | https://doi.org/10.1006/viro.1997.8648 | |
dc.subject | Immunity | |
dc.subject | Immunology and Infectious Disease | |
dc.subject | Immunology of Infectious Disease | |
dc.subject | Infectious Disease | |
dc.subject | Virology | |
dc.title | Recognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones | |
dc.type | Journal Article | |
dc.source.journaltitle | Virology | |
dc.source.volume | 234 | |
dc.source.issue | 2 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/infdis_pp/302 | |
dc.identifier.contextkey | 10843618 | |
html.description.abstract | <p>We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-1-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes.</p> | |
dc.identifier.submissionpath | infdis_pp/302 | |
dc.contributor.department | Department of Medicine, Division of Infectious Diseases and Immunology | |
dc.contributor.department | Center for Infectious Disease and Vaccine Research | |
dc.source.pages | 383-6 |