Show simple item record

dc.contributor.authorGreen, Sharone
dc.contributor.authorKurane, Ichiro
dc.contributor.authorPincus, Steven
dc.contributor.authorPaoletti, Enzo
dc.contributor.authorEnnis, Francis A.
dc.date2022-08-11T08:09:09.000
dc.date.accessioned2022-08-23T16:19:37Z
dc.date.available2022-08-23T16:19:37Z
dc.date.issued1997-08-04
dc.date.submitted2017-10-02
dc.identifier.citationVirology. 1997 Aug 4;234(2):383-6. <a href="https://doi.org/10.1006/viro.1997.8648">Link to article on publisher's site</a>
dc.identifier.issn0042-6822 (Linking)
dc.identifier.doi10.1006/viro.1997.8648
dc.identifier.pmid9268170
dc.identifier.urihttp://hdl.handle.net/20.500.14038/35093
dc.description.abstractWe examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-1-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=9268170&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttps://doi.org/10.1006/viro.1997.8648
dc.subjectImmunity
dc.subjectImmunology and Infectious Disease
dc.subjectImmunology of Infectious Disease
dc.subjectInfectious Disease
dc.subjectVirology
dc.titleRecognition of dengue virus NS1-NS2a proteins by human CD4+ cytotoxic T lymphocyte clones
dc.typeArticle
dc.source.journaltitleVirology
dc.source.volume234
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/infdis_pp/302
dc.identifier.contextkey10843618
html.description.abstract<p>We examined nine dengue virus-specific human CD4+ CD8- cytotoxic T lymphocyte (CTL) clones for protein recognition, using recombinant vaccinia viruses which contain genes coding for dengue virus proteins. These clones were established from peripheral blood mononuclear cells of a donor previously immunized with a live-attenuated experimental dengue-1 vaccine. Of nine CD4+ T cell clones, seven were dengue-1-specific and two were dengue-1-dengue-3 cross-reactive. Four dengue-1-specific clones and one dengue-1-dengue-3 cross-reactive clone recognized epitopes within the NS1 or NS2a proteins. Analysis of human leukocyte antigen (HLA) restriction revealed that three dengue-1-specific clones are HLA-DR1-restricted and one dengue-1-dengue-3 cross-reactive clone is HLA-DPw3-restricted. These results indicate that NS1 and NS2a proteins as well as C, E, and NS3 proteins reported earlier contain one or more epitopes recognized by dengue virus-specific human CD4+ T lymphocytes.</p>
dc.identifier.submissionpathinfdis_pp/302
dc.contributor.departmentDepartment of Medicine, Division of Infectious Diseases and Immunology
dc.contributor.departmentCenter for Infectious Disease and Vaccine Research
dc.source.pages383-6


This item appears in the following Collection(s)

Show simple item record