Induction of Japanese encephalitis virus-specific cytotoxic T lymphocytes in humans by poxvirus-based JE vaccine candidates
Mason, Peter W.
Shope, Robert E.
Smucny, John J.
Hoke, Charles H. Jr
Ennis, Francis A.
UMass Chan AffiliationsDepartment of Medicine, Division of Infectious Diseases and Immunology
Center for Infectious Disease and Vaccine Research
Document TypeJournal Article
Immunology and Infectious Disease
Immunology of Infectious Disease
MetadataShow full item record
AbstractPoxvirus-based recombinant Japanese encephalitis (JE) vaccine candidates, NYVAC-JEV and ALVAC-JEV, were examined for their ability to induce JE virus-specific cytotoxic T lymphocytes (CTLs) in a phase I clinical trial. These vaccine candidates encoded the JE virus premembrane (prM), envelope (E) and non-structural 1 (NS1) proteins. The volunteers received subcutaneous inoculations with each of these candidates on days 0 and 28, and blood was drawn 2 days before vaccination and on day 58. Anti-E and anti-NS1 antibodies were elicited in most vaccinees inoculated with NYVAC-JEV and in some vaccinees inoculated with ALVAC-JEV. Peripheral blood mononuclear cells (PBMCs) obtained from approximately one half of vaccines showed positive proliferation in response to stimulation with live JE virus. Cytotoxic assays demonstrated the presence of JE virus-specific CTLs in in vitro-stimulated PBMCs obtained from two NYVAC-JEV and two ALVAC-JEV vaccinees. Cell depletion tests using PBMCs from one NYVAC-JEV recipient indicated that the phenotype of CTLs was CD8+CD4-.
SourceVaccine. 1998 May;16(8):842-9. Link to article on publisher's website
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/35103
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