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    Dengue virus-specific, human CD4+ cytotoxic T lymphocytes generated in short-term culture

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    Authors
    Zivny, Jaroslav
    Kurane, Ichiro
    Tacket, Carol O.
    Edelman, Robert
    Ennis, Francis A.
    UMass Chan Affiliations
    Department of Medicine, Division of Infectious Diseases and Immunology
    Center for Infectious Disease and Vaccine Research
    Document Type
    Journal Article
    Publication Date
    1993-01-01
    Keywords
    Immunity
    Immunology and Infectious Disease
    Immunology of Infectious Disease
    Infectious Disease
    Virology
    
    Metadata
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    Link to Full Text
    https://doi.org/10.1089/vim.1993.6.143
    Abstract
    We previously reported cytotoxic activity of dengue virus-specific CD4+ CD8- T cell clones established in long-term in vitro culture. In the present experiments we tried to determine whether dengue virus-specific CD4+ CD8- CTL3 are present in short-term bulk cultures. Peripheral blood mononuclear cells (PBMC)3 from a donor who had been immunized with an experimental live attenuated dengue 1 vaccine 8 months earlier were used. PBMC were incubated with noninfectious dengue 1 antigen (Ag)3 for 7 days, and were examined for dengue 1-specific cytotoxic activity. PBMC cultured with dengue 1 Ag lysed autologous lymphoblastoid cell line (LCL)3 pulsed with noninfectious dengue 1 Ag, but did not lyse LCL pulsed with Ag of other dengue serotype, West Nile virus, or yellow fever virus, or control Ag. Treatment of cultured PBMC with monoclonal antibody to CD3 or CD4 and complement abrogated the cytotoxic activity but treatment with a monoclonal antibody to CD8 and complement did not. A time course study showed that dengue 1 Ag-specific CTL were first detected in 5 day cultures. Lysis of target cells by these CD4+ CTL were restricted by HLA class II, and HLA DQw1 and HLA DRw52 were determined to be the restriction molecules. These results indicate that dengue virus-specific CD4+ CD8- CTL are generated in short-term bulk cultures as well as in long-term-cultured cell lines, and support the concept that CD4+ CTL may be generated in vivo during infection.
    Source
    Viral Immunol. 1993 Summer;6(2):143-51. Link to article on publisher's site
    DOI
    10.1089/vim.1993.6.143
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/35116
    PubMed ID
    8105794
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1089/vim.1993.6.143
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