UMass Chan Affiliations
Department of Medicine, Division of Infectious Diseases and ImmunologyDocument Type
Journal ArticlePublication Date
2010-02-01Keywords
Actinobacillus actinomycetemcomitansCell Line
Chronic Periodontitis
Fusobacterium nucleatum
Humans
Immune Evasion
Interleukin-8
Kidney
Lipid A
NF-kappa B
Nod1 Signaling Adaptor Protein
Nod2 Signaling Adaptor Protein
Peptidoglycan
Porphyromonas gingivalis
Immunology and Infectious Disease
Metadata
Show full item recordAbstract
In bacterial infection, Nucleotide-binding Oligomerization Domain (NOD) 1 and NOD2 induce innate immune responses by recognizing fragments of the bacterial component peptidoglycan (PGN). To determine the roles of these receptors in detection of periodontal pathogens, we stimulated human embryonic kidney cells expressing NOD1 or NOD2 with heat-killed Porphyromonas gingivalis, Aggregatibacter actinomycetemcomitans, and Fusobacterium nucleatum or their soluble PGNs (sPGNs). All bacteria and their sPGNs could stimulate activation of NF-kappaB. However, there were differences in NOD1- and NOD2-stimulatory activities among the species of bacteria. P. gingivalis showed weaker NOD1- and NOD2-stimulatory activities than did other bacteria. These differences in activities were confirmed by production of interleukin-8 from oral epithelial cells stimulated with sPGNs. These findings indicate that both NOD1 and NOD2 might be involved in the recognition of periodontal pathogens, and that the weak NOD-stimulatory property of P. gingivalis might be helpful for survival in the periodontal pocket.Source
J Dent Res. 2010 Feb;89(2):186-91. Epub 2009 Dec 29. Link to article on publisher's siteDOI
10.1177/0022034509354843Permanent Link to this Item
http://hdl.handle.net/20.500.14038/35169PubMed ID
20040739Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1177/0022034509354843